硼替佐米对K562细胞株粘附分子ICAM-1表达的影响

目的:探讨蛋白酶体抑制剂硼替佐米(万珂,Valcade)对白血病K562细胞株细胞问粘附分子(ICAM-1)表达的影响。方法:用含10?S的RPMI1640培养液体外培养K562细胞,分别用0、10、20、30、50、100 nmol/L的硼替佐米干预K562细胞6h后收集,用反转录聚合酶链反应(RT-PCR)分析K562细胞ICAM-1表达的变化。并用20 nmol/L浓度硼替佐米作用K562细胞株不同时间(0,6,12,24,48h)分析K562细胞ICAM-1表达的变化。结果:硼替佐米明显抑制K562细胞ICAM-1的表达(P<0.05),在硼替佐米浓度为0～20 nmol/L时成正比关系,当浓度>20 nmol/L各组差异无显著性。以20 nmol/L浓度作用K562细胞株不同时间后,ICAM-1的表达较作用前明显下降.并且这种效应持续存在。结论:K562细胞株ICAM-1基因表达异常增高,硼替佐米可抑制其表达量。

Study on Effects of Bortezomib on Expression of ICAM-1 of K562 Cells

Objective:To investigate the effects of proteasome inhibitor bortezomib(Valcade,PS-341)on the ex- pression of intercellular adhesion molecule-1(ICAM-1)of K562 cells.Methods:KS62 cells were incubated with RP- MI1640 and exposed to bortezomib in different concentration(0,10,20,30,50,100 nmol/L)and different time(0,6,12, 24,48 h)respectively.Then the expression of ICAM-1 was detected by RT-PCR.Results:The expression of ICAM-1 was decreased significantly after bortezomib treatment.The inhibitory effect on ICAM-1 was positively related with drug concentration when the drug concentration was increased from 0 nmol/L to 20 nmol/L,whereas it remained unchange- able when the drug concentration exceeded 20 nmol/L.And another results showed that the inhibitory effect was lasted for 48 h at least.Conclusion:The expression of ICAM-1 in K562 cells is increased,which can be inhibited by bortezomib.