| Apolloon反义寡核苷酸对K562细胞增殖和凋亡的影响 |
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| 引用本文： | 刘媛媛,贾秀红,李建厂,韩兆东,谢绍华.Apolloon反义寡核苷酸对K562细胞增殖和凋亡的影响[J].肿瘤防治研究,2011,38(6):636-638. |
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| 作者姓名： | 刘媛媛 贾秀红 李建厂 韩兆东 谢绍华 |
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| 作者单位： | 1. 256603山东滨州，滨州医学院附属医院儿科，2.中心实验室 |
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| 摘 要： | 目的探讨Apollon反义寡核苷酸（antisense oligonucleotide，ASODN）对白血病细胞K562增殖及凋亡的影响。方法设计合成特异性的Apollon硫代磷酸ASODN及其对照错义寡核苷酸（missense oligonucleotide，MSODN），脂质体介导转染K562细胞后，采用MTT法检测K562细胞增殖，Annexin V-FITC法检测细胞的凋亡。结果Apollon反义寡核苷酸对K562细胞的生长抑制呈浓度-时间依赖性。Apollon ASODN在终浓度为600 nmol/L作用K562细胞时，能明显抑制其增殖，K562细胞凋亡率显著增加（P<0.01）。结论Apollon 反义寡核苷酸可下调Apollon基因表达，有效抑制K562细胞的增殖，并促进K562细胞凋亡。
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| 关 键 词： | Apollon 反义寡核苷酸 K562 细胞增殖 凋亡 |
| 收稿时间： | 2010-07-26; |
Effects of Apollon Antisense Oligonucleotide on Proliferation and Apoptosis of K562 Cells |
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| LIU Yuan-yuan,JIA Xiu-hong,LI Jian-chang,HAN Zhao-dong,XIE Shao-hua.Effects of Apollon Antisense Oligonucleotide on Proliferation and Apoptosis of K562 Cells[J].Cancer Research on Prevention and Treatment,2011,38(6):636-638. |
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| Authors: | LIU Yuan-yuan JIA Xiu-hong LI Jian-chang HAN Zhao-dong XIE Shao-hua |
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| Institution: | 1.Department of Pediatrics, Affiliated Hospital of Binzhou Medical University, Binzhou 256603, China，2.Central Laboratory |
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| Abstract: | Objective To investigate effects of Apollon antisense oligonucleotide(ASODN) on the proliferation and apoptosis of human leukemia(K562) cells.Methods Specific phosphorothioate ASODN and missense oligonucleotide(MSODN) of Apollon mRNA were synthesized and transfected into K562 cells following cationic liposome.The proliferation inhibition of K562 cells was assessed by MTT.The apoptosis rate was detected by Annexin V-FITC.Results Apollon antisense oligonucleotide inhibited the cell proliferation in a dose and... |
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| Keywords: | Apollon Antisense oligonucleotide K562 Cell proliferation Apoptosis |
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