蛇毒精氨酸酯酶Agkihpin对人鼻咽癌CNE-2细胞系MRP1表达的影响

目的探讨蛇毒精氨酸酯酶Agkihpin对人鼻咽癌CNE-2细胞系中多药耐药相关蛋白MRP1表达的影响，并试图阐明Agkihpin抑制CNE-2细胞的机制。方法用不同浓度的Agkihpin处理细胞72 h后，应用免疫细胞化学、Western blot、RT-PCR法检测MRP1在CNE-2细胞中的表达。结果不同浓度Agkihpin作用CNE-2细胞72 h后MRP1表达均降低，并呈现出一定的浓度依赖效应，显示Agkihpin可显著下调CNE-2细胞中MRP1表达。各加药组与不加Agkihpin组比较，差异具有统计学意义（P<0.05）。结论在人低分化鼻咽癌CNE-2细胞系中，Agkihpin能抑制MRP1的表达，并且随浓度的增大抑制作用增加，这可能是Agkihpin能降低鼻咽癌细胞活力的原因之一；Agkihpin抑制MRP1的表达提示在一定程度上可提高肿瘤细胞对化疗药物的敏感度。

Effect of Agkihpin-an Arginine Esterase from Venom on Expression of MRP1 in CNE-2 Cells

Objective To explore the effect of Agkihpin on the expression of multidrug resistance associated protein 1(MRP1) in nasopharyngeal carcinoma CNE-2 cell line,and try to describe the mechanism of Agkihpin inhibiting CNE-2.Methods The cultured cells were treated with different concentrations of Agkihpin for 72 h.The expression level of MRP1 in CNE-2 cell line was assayed by immunocytochemistry,Western blot and RT-PCR.Results Comparing with Agkihpin absent group,the MRP1 expression levels in CNE-2 cells were re...