慢性乙型肝炎肝组织ICAM-1 mRNA和ICAM-1表达及意义

目的探讨肝组织间粘附分子-1(ICAM-1)表达在慢性乙型肝炎(CHB)发病机理中的作用。方法用原位杂交和免疫组织化学技术检测11例正常人和50例慢性HBV感染者肝内ICAM-1 mRNA和ICAM-1表达情况。结果正常人和慢性无症状HBsAg携带者肝细胞无ICAM-1mRNA和ICAM-1表达,CHB患者肝细胞ICAM-1 mRNA和ICAM-1表达增强,阳性肝细胞多分布在汇管区周围和腺泡内炎症坏死区域;重度CHB患者肝细胞ICAM-1 mRNA和ICAM-1表达显著强于中、轻度CHB患者(P<0.05);肝细胞ICAM-1表达强度与肝组织炎症活动度呈显著正相关,p<0.01;肝细胞ICAM-1表达强的患者肝功能显著差于ICAM-1表达弱者,P<0.05。结论肝细胞ICAM-1表达在慢性乙型肝炎肝细胞坏死中起重要作用,肝细胞ICAM-1表达水平能较好反映其肝损害程度和肝功能状况。

Hepatic Expression of ICAM-1 mRNA and ICAM-1 in Patients with Chronic Viral Hepatitis B and its Significance

Objective To study the role of hepatic ICAM-1 expression in the pathogenesis of chronic viral hepatitis B (CHB). Methods Hepatic Expressions of ICAM-1 mRNA and ICAM-1 were studied from 11 healthy persons and 50 patients with chronic HBV infection by in situ hybridization and immunohistochemical method. Results In healthy persons and chronic asymtomic HBsAg carriers, no ICAM-1 mRNA and ICAM-1 expressions were found in hepatocytes; in CHB, ICAM-1 mRNA and CIAM-1 were strongly expressed in hepotocytes. Hepatocytes with ICAM-1 mRNA or ICAM-1 expression were mostly located in periportal and intra-acinar areas of inflammation and necrosis. ICAM-1 mRNA and ICAM-1 expressions in hepatocytes were significantly stronger in severe CHB than in moderate to mild CHB, (P<0.05). The levels of ICAM-1 expression in hepatoeytes positively correlated with the inflammatory activities in liver, (P<0.01). Liver function was significantly worse in patients with enhanced expression of ICAM-1 than in cases with weak expression of ICAM-1 on hepatocytes, (P<0.05). Conclusion In CHB, ICAM-1 expression in hepatoeytes plays an important role in the pathogenesis of hepatoeellular necrosis, and the levels of ICAM-1 expression may reflect the degree of liver damage and the condition of liver function.