脐带间充质干细胞向汗腺样细胞分化后的表型变化

目的：比较人脐带间充质干细胞（hUC-MSCs）向汗腺样细胞诱导前后表型特征的变化.方法：对hUC-MSCs进行分离、培养、鉴定,通过显微镜观察、免疫细胞化学、流式细胞术等方法比较hUC-MSCs经汗腺分化诱导培养液培养前后细胞形态变化及癌胚抗原（CEA）、角蛋白7（CK7）、CK8、CK14、CK18、CK19表达的差异.结果：hUC-MSCs向汗腺样细胞诱导前呈梭形,呈成纤维细胞样;流式细胞仪检测发现CD29、CD90表达阳性;而CD34、CEA、CK14、CK19表达阴性.经汗腺诱导培养基诱导后hUC-MSCs分化为外形肥大、不规则、类似铺路石样的细胞,聚集性增殖;免疫细胞化学检测显示CK7、CK8、CK18抗原表达阳性;流式细胞仪检测结果显示CEA、CK14、CK19的阳性表达率分别为77.98%,48.47%,20.85%.结论：hUC-MSCs经过汗腺分化诱导培养基培养后能够分化为表达汗腺细胞标记物抗原的汗腺样细胞.

Phenotypic changes of human umbilical cord derived mesenchymal stem cells after induction to sweat gland-like cells

Objective：To observe the phenotype of human umbilical cord derived mesenchymal stem cells （hUC- MSCs） before and after being induced to sweat gland-like cells. Methods： bUC-MSCs were isolated,cultured and identified. Phenotypic changes in uninduced and induced hUC-MSCs were examined with morphological observation, immunocytochemical and flow cytometry analysis for CEA, CK 14 and CK 19. Results：Original hUC-MSCs appeared to have fibroblast-like morphology, with positive expression of CD29, CD90, but with negative CD34, CEA, CK14, CK19 expression, and could be differentiated into adipose cells （Oil Red-O staining-positive）, and osteogenic cells （positive for alkaline phosphatase （AP）. After being cultured in sweat gland-induction medium for 21 days, hUC-MSCs could express sweat gland markers （CEA, CK14, CK19）, and possessed sweat gland-like morphology. Conclusion：The results revealed that hUC-MSCs could differentiate into sweat gland-like cells after culturing in induction medium, and they could be considered a potential source of stem cells for the restoration of destroyed sweat glands.