树突状细胞体内回输对烫伤小鼠过度炎症反应的调控作用

目的:观察树突状细胞(DCs)与调节性树突状细胞(CD11clowCD45RBhigh DCs)体内回输对烫伤所致腹腔炎症反应的影响.方法:采用15%体表面积Ⅲ度烫伤(99.0±0.5℃)沸水中8 s]小鼠模型.伤后即刻腹腔注射林格液(1 ml/只)抗休克治疗(烫伤组,n=21)或者腹腔注射加入自小鼠脾脏分离纯化而得...

Effect of administration of dendritic cells on inflammatory response in scald mice

Objective： To investigate the effect of dendritic cells （DCs） and regulatory dendritic cells （CD11c ^low CD45RB^high DCs） on thermal injury-induced inflammatory cytokine production through single intraperitoneal injection of DCs and CD11c^low CD45RB^high DCs to scald mice. Methods： Mice were subjected to a 15% total body surface area （TBSA） scald injury on the back. Both sham- and burn-injured mice received 1.0 ml of Ringer＇s solution for resuscitation. CD11c^low CD45RB^high DCs and DCs was added to lactated Ringer＇s solution （1 ml/mouse） in treatment groups. Animals of all groups were sacrificed at post burn hours （PBH） 12, 24 and 48 respectively, and peritoneal fluid samples were harvested aseptically and stored immediately at - 70 ℃ until use for measurement of cytokine levels. Spleens were used to procure CD11c^low CD45RB^high DCs and DCs. Result： IL-6 levels in peritoneal fluid in CD11c^low CD45RB^high DCs group （296.57±11.40）, （152.89±6.03）, （53.18±3.25） pg/ml] and DCs group （602.44±20. 64）, （304.35±9.46）, （118.30±8.90）pg/ml] were significant decreased compared with scald group （896.21±24.63） ,（451.74±13.22） ,（199.02±7.83） pg/ml] at PBH 12, 24 and 48 （all P〈0. 01）. Similarly, MCP-1 levels in peritoneal fluid in CD11c^low CD45RB^high DCs group （1 308.40±87.90）, （472.00±29.37）, （61.29±5.20） pg/ml] and DCs group （1 708.36±103.22）, （789.23±49.02）, （86.33±6.11） pg/ml] were markedly lowered compared with untreated mice （2 019.59± 129.66）, （1 067.90±65.88）, （152.87± 14.28） pg/ml] at PBH12, 24 and 48 （all P〈0.01）. Moreover, TNF-a levels in peritoneal fluid in both CD11c^low CD45RB^high DCs and DCs treated group were significantly lower than that in untreated mice （81.63 ±6.02）, （54.74±4.01）, （29.45±2.51） pg/ml] at PBH 12, 24 and 48 （P〈0.01）. In addition, IL-6, MCP-1 and TNF- a levels were markedly lowered in CD11c^low CD45RB^high DCs group compared with DCs group at various intervals （P 〈0.01） Conclusion： Naturally existed CD11c^low CD45RB^high DCs and DCs might effectively inhibit excessive inflammation in mi ce with major burns.