丹参对右旋葡聚糖硫酸钠诱导小鼠结肠炎的疗效观察

目的 :评价丹参预防及治疗右旋葡聚糖硫酸钠 (DSS)结肠炎小鼠的有效性。方法 :2 0只正常小鼠随机分为两组 ,饮用 DSS7d,同时预防组用丹参 ,对照组用 0 .85 %氯化钠溶液。另 2 0只 DSS诱导的结肠炎小鼠随机分为两组 ,治疗组用丹参 ,对照组用 0 .85 %氯化钠溶液 7d。用疾病活动指数 (DAI)、组织学评分和马休斯猩红蓝(MSB)纤维素染色检测微血栓以评价疗效。结果 :丹参在预防组部分降低微血栓的形成 ,对照组 10例有 6例微血栓阳性 ,预防组 3例阳性。丹参治疗组与对照组的 DAI、直肠、横结肠组织学评分分别为 0 .4 5、0 .4 8(P>0 .0 5 ) ,1.36、1.76 (P<0 .0 5 ) ,1.35、1.6 0 (P<0 .0 5 )。结论 :丹参可能部分抑制微血栓形成和减轻 DSS结肠炎小鼠结肠炎症 ,提示丹参用于溃疡性结肠炎治疗也可能有效。

Preventive and Therapeutic Effect of Salvia Miltiorrhiza Bge on Colitis of Mice Induced by Dextran Sulphate Sodium

Objective: To evaluate the preventive and therapeutic effect of Salvia miltiorrhiza Bge on colitis of mice induced by dextran sulphate sodium (DSS). Methods: The normal mice (n=20) received DSS orally for 7 days were randomly divided into two groups: the preventive group with Salvia miltiorrhiza Bge and the control group with normal saline. The colitis mice induced by DSS (n=20) were divided into two groups: the therapeutic group with Salvia miltiorrhiza Bge and the control group with normal saline for 7 days. The preventive and therapeutic effect of Salvia miltiorrhiza Bge was assessed by disease activity index (DAI), histologic score and MSB fibrin staining to identify microvascular thrombi. Results: Microvascular thrombi in the preventive group partly decreased compared with that in the control group. Microvascular thrombi were positive in 6 of 10 control mice and 3 of 10 in the preventive group. DAI, rectal and transverse colonic histological score in the therapeutic group and the control group were 0.45 and 0.48(P>0.05),1.36 and 1.76(P<0.05),1.35 and 1.60 (P<0.05) respectively. Conclusion: Salvia miltiorrhiza Bge could partly inhibit the formation of the microvascular thrombosis and effectively reduce the colonic and rectal inflammation of DSS-induced colitis in the mice. It suggested that Salvia miltiorrhiza Bge also be effective in ulcerative colitis.