X射线诱发外周血淋巴细胞TCR基因突变研究

目的 用培养法研究X射线诱发的人外周血淋巴细胞TCR 基因突变情况。方法 以不同剂量(0~8 Gy) 的X射线照射新鲜分离的健康成人外周血淋巴细胞, 植物血凝素、白细胞介素2(IL-2)协同刺激培养7 d, 流式细胞术检测TCR基因突变频率(TCR MF),并拟合剂量效应关系。结果 随着照射剂量的增加,TCR基因突变频率随之上升, 最佳拟合曲线为二次多项式模型。结论 TCR 基因突变可作为辐射生物剂量计,用于急性辐射照射生物剂量的估算。

X-ray induced TCR gene mutation of peripheral blood lymphocytes

Objective To study the TCR gene mutation in peripheral blood lymphocytes induced by X-ray exposure using cultured lymphocytes cloning method.Methods Freshly isolated peripheral lymphocytes from healthy adult donors were irradiated with X-ray in doses ranging from 0 to 8 Gy and cultured with interleukin2 and phytohemagglutinin for 7 days. The mutant frequencies of TCR gene (TCR MF) were detected by flow cytometry and the dose response curves were fitted.Results TCR MF increased with the dose going up. An aquadratic polynomial dose response model was fitted.Conclusion TCR gene mutation could which serve as a potential biological dosimeter. It might be applied for the estimation of biological dose in emergency exposure.