日本血吸虫感染动物血清中循环抗体（CAb）及循环抗原（CAg）水平的动态研究

应用膜片固相抗原免疫酶测定法（Ｄ－ＩＥＡ）和ＥＬＩＳＡ检测日本血吸虫感染家兔血清中ＣＡｂ和ＣＡｇ水平时，高峰分别在感染后１０～１２周和１０周，ＣＡｂ和ＣＡｇ水平均与感染度和感染期密切相关。实验结果表明，感染宿主血清中ＣＡｂ／ＣＡｇ水平的消长规律并不一致，宿主在感染中期（感染后１０～２０周）显示ＣＡｂ检测为优势，在感染早期（感染后１０周内）则以ＣＡｇ检测为优势。本项研究可为选用适宜的ＣＡｂ／ＣＡｇ检测途径，提供依据。应用改进方法Ｄ－ＩＥＡ检测血吸虫感染者血清中特异性抗体时，可取得满意效果。

Studies on the Dynamic Changes of Circulating Antibody (CAb)and Circulating Antigen (CAg) Levels in Sera from Animals Infected with Schistosoma japonicum

Disc-immunoenzymatic assay(D-IEA) and enzyme-linked unmunosorbent assay(ELISA) were performed to detect the dynamics of CAb CAg levels in sera form rabbits with Schistosoma japonicum, both peaks appeared at the 10th to 12th and 10th weeks after infection respectively. The levels of CAb and CAg were closely correlated with the intensity and duration of infection .The results indicated that the dynamic changes of circulating antibody and antigen levels were markedly different,the superiority of CAb detection appeared in the middle period of infection (namely the 10th to 20th weeks after infection)and that of CAg detection showed in the early period(namely within ten weeks).At the same time the results provided a seientific basis for selecting the suitable detection ways of CAb and CAg, and D-IEA was good for the detection of specific circulating antibody in sera from the in fected rabbits.