Avastin抑制兔角膜新生血管的实验研究

目的探讨Avastin对角膜新生血管（NV）的抑制作用。方法日本大耳白兔20只，以角膜缝线法制作角膜新生血管模型，观察4周。在角膜缝线1周后，实验组以浓度为4mg／mL的Avastin溶液滴眼，对照组以注射用水滴眼，均为每日2次，共28d。记录角膜新生血管出现的时间、生长的长度和数量。结果两组角膜新生血管的出现时间分别为（3．43±0．53）d和（3．14±0．69）d（P〉0．05）；在缝线后14d、21d、28d，实验组角膜新生血管的面积均明显小于对照组（P〈0．05）。且实验组角膜新生血管在用药后明显变细。两组角膜荧光素染色在观察期间均未发现明显着色。结论Avastin滴眼能够有效抑制角膜新生血管的生长，并能在一定程度上使已有的新生血管消退，且未发现明显的毒副作用。

Inhibition of corneal neovascularization by Avastin in rabbits

Objective To evaluate the effect of Aavastin to inhibit experimental corneal neovascularization. Methods Twenty rabbits were sutured on the superior cornea. Animals were randomly divided into 2 groups. After 7 days, 10 eyes in the treatment group were given Aavastin drops(4 rog/mi) twice daily, whereas 10 eyes in the control group received placebo (normal saline drops twice daily). The occurrence and developement of CNV was observed on the 3, 7, 14, 21,28 days after suturing by slit-lamp microscope, and the area of CNV was calculated. Fluorescein staining was detected for the integrity of corneal epithelium. Results The occurrence of CNV in group 1 was (3.43±0.53)d and group 2 (3.14±0.69)d. No significant difference in the occurrence time of CNV was found between group 1 and group 2. The CNV areas of group 1 were significantly smaller than that of group 2 at every time poin(P<0.05). The diameter of CNV was significantly smaller after Avastin treatment. Conclusion Avastin can inhibit and restrain corneal neovascularization at some degree without significant side effects.