Fe_3O_4磁纳米介导磁感应治疗Wistar大鼠Walker-256肿瘤

目的探讨Fe3O4磁性纳米微球磁感应对Wistar大鼠Walker-256肿瘤的治疗作用。方法荷瘤鼠随机分为NS组（注入生理盐水而不接受磁场处理）、MF组（注入磁流体而不接受磁场处理）、MFH1组（注入磁流体在交变磁场中治疗1次）、MFH2组（治疗2次）和MFH3组（治疗3次）,共5组。治疗温度控制在50～55℃,分别观察肿瘤体积抑制率和生存期,以及肿瘤的病理改变。结果MFH2组和MFH3组的肿瘤在2周内有明显的肿瘤体积抑制作用,肿瘤体积抑制率分别为（65.0±3.5）%、（71.6±4.2）%。与NS组（35.5±5.6）d比较,MFH3组（41.9±6.5）d和MFH2组（42.1±5.0）d的生存期延长,差异有统计学意义（q=4.386,P〈0.05;q=4.558,P〈0.05）;MFH1组（39.0±5.3）d和MF组（36.1±6.6）d的生存期差异无统计学意义（q=2.406,P〉0.05）。NS、MF组肿瘤表面光滑,小灶性坏死区;癌细胞密度大,核仁明显,易见核分裂相。MFH2和MFH3组瘤内大片坏死残留的无结构红染物质,或空洞,部分胞核有固缩、碎裂及崩解。结论Fe3O4纳米磁流体介导的磁热疗能抑制大鼠Walker-256皮下肿瘤增殖。

Therapeutic Effect of Fe3O4 Nanometer Magnetic Induction Hyperthermia on Walker-256 Tumor in Wistar Rats

Objective To study the therapeutic effect of F3O4 nanometer magnetic induction hyperthermia under alternating magnetic field on subcutaneous tumor model of carcinomatous Walker-256 cells in Wistar rats. Methods After subcutaneous tumor modesl were established, Wistar rats with the tumor tissues were randomly divided into 5 groups to receive injections of normal saline （NS） without hyperthermia, magnetic fluid （MF） without hyperthermia or magnetic fluid with hyperthermia for one time （ MFH1 ） , for two times （MFH2） , or three times （ MFH3 ）. The temperature was controlled at 50 - 55 ℃. The rate of volume inhibition and survival time of the tumor, and the pathological changes were observed. Results The tumor volume was reduced significantly in the MFH2 and MFH3 groups [（65.0 ±3.5）% and （71.6 ±4.2）% respectively]. The survival time of the tumor in the two groups were significantly longer than that in the NS group [（42.1 ±5.0） d and （41.9±6.5） d vs （35.5±5.6） d; q=4.386, P〈0.05; q= 4. 558, P 〈 0. 05, respectively]. No significant difference was detected in the survival time of the tumor between the MFH1 and MF groups [ （39.0 ± 5.3 ） d vs （36. 1 ± 6.6） d, q = 2. 406, P 〉 0. 05 ]. Pathological examination showed smooth tumor surface and small necrotic areas in the NS and MF groups, in which the cancer cells were thick, and stage of mitosis was obviously observed. In the MFH2 and MFH3 groups, large necrosis areas and red-dyed remnant or cavity, and shrank, split and dissolved nuclei were visible. Conclusions Fe3O4 nanometer magnetic fluid hyperthermia can inhibit the proliferation of subcutaneous Walker-256 tumor model in Wistar rats.