HPLC法测定复方皂矾丸中西洋参的含量

目的:建立复方皂矾丸中西洋含量测定测方法.方法:采用高效液相色谱法同时测定西洋参中人参皂苷Rg1、人参皂苷Re和人参皂苷Rb1的含量.结果:高效液相色谱人参皂苷Rg1的线性范围是0.63～5.06 μg(r＝0.999 9),平均加样回收率101.12%(RSD＝2.14%),人参皂苷Re的线性范围是1.05～8.4 μg(r＝0.999 8),平均加样回收率100.71%(RSD＝1.57%),人参皂苷Rb1的线性范围是2.468～19.744μg(r＝0.999 7),平均加样回收率97.22%(RSD＝1.92%).结论:该方法操作简便、结果准确、重现性好,可用于复方皂矾丸中西洋参的含量测定.

Determination of American Ginseng in Compound Zaofan Pills

Objecitve： To establish a method to determine the content of American ginseng in Compound Zaofan Pills. Methods： HPLC was used to determine the content of ginsenoside Rg1 , Re and Rb1 of the American ginseng. Results ： This method had good lin earity in the range of 0. 63 - 5.06 μg（ r = 0. 999 9 ）, and the average recovery was 101.12% with RSD of 2. 14% for ginsenoside Rg1. It had good linearity in the range of 1.05 N 8.4μg（ r = 0. 999 8） , and the average recovery was 100. 71% with RSD of 1.57% for ginsenoside Re. It had good linearity in the range of 2.47 - 19. 74μg（ r=0.9997）, and the average recovery was 97.22% with RSD of 1.92% for ginsenoside Rbl. Conclusion： The method was shown to be convenient, accurate with good repeatability, so it was suitable to determine the content of American ginseng in Compound Zaofan Pills.