氯沙坦对实验兔颈动脉粥样硬化和血清ICAM-1、VCAM-1的影响

目的:观察氯沙坦对动脉粥样硬化程度和黏附分子的影响.方法:将24只雌雄各半新西兰白兔随机均分为对照组、高脂组、治疗组.对照组喂普通饲料,高脂组喂以高脂饲料,治疗组为高脂饲料加用氯沙坦25mg/(kg·d).分别于实验开始前、开始后第4、8、12周清晨空腹取各组实验动物耳中央静脉血0.5ml,分别测定并比较各组空腹状态下...

Effect of Losartan on Carotid Atherosclerosis and Serum Levels of ICAM-1,VCAM-1 in Rabbits

Objective To study the effects of losartan on carotid atherosclerosis,serum intercellular adhesion molecule-1(ICAM-1) and vascular cell adhesion molecule-1(VCAM-1).Methods Twenty-four New Zealand rabbits were randomly and equally assigned to three groups: control group fed with normal diet,high-cholesterol group fed with high-cholesterol diet alone and Losartan-treated group fed with high-cholesterol diet and treated with Losartan 25mg/(kg·d).0.5ml blood from central veins of the ears in the morning was collected before the experiment and at 4,8,and 12 wks after the experiment respectively.The animals were fasted for 12 hours before the blood collection.Serum ICAM-1 and VCAM-1 were measured with ELISA.All the rabbits were killed by depletion method after 12 wks.Bilateral carotids were separated.The area of CAP with sudan III staining and the tunica media with hematoxylin-eosin staining were measured.Results The levels of serum ICAM-1 and VCAM-1 in high-cholesterol group were higher than control group at each timepoint after the experiment.Compared with high-cholesterol group,the levels of serum ICAM-1 and VCAM-1 in treatment group were significantly lower 8,and 12 wks(P<0.01).The atherosclerotic plaque was less obvious in treatment group than which in high-cholesterol group.Observed with hematoxylin-eosin staining,the tunica intima thickened in high-cholesterol group and which in treatment group was less obvious.Conclusion Losartan could significantly inhibit the development of the carotid artery atherosclerosis and prominently reduce the expression of serum ICAM-1 and VCAM-1.