HPLC法测定益心酮片中五种成分的含量

目的:建立HPLC同时测定益心酮片中牡荆素-4″-O-葡萄糖苷、牡荆素鼠李糖苷、牡荆素、芦丁、金丝桃苷的含量。方法:采用HPLC法,色谱柱为Sepax HP C18柱(4.6 mm×250 mm,5μm);流动相:A为乙腈-四氢呋喃(20∶1),B为0.5%甲酸溶液,采用梯度洗脱;0~10 min,A为14%~17%,B为86%~83%;10~26 min,A为17%~18%,B为83%~82%;26~28 min,A为18%~14%,B为82%~86%;检测波长360 nm,流速1.0 mL.min-1;柱温32℃。结果:该方法加样回收率牡荆素-4″-O-葡萄糖苷为99.61%(RSD=0.64%),牡荆素鼠李糖苷为99.30%(RSD=0.49%),牡荆素为98.97%(RSD=1.03%),芦丁为99.45%(RSD=0.85%),金丝桃苷为99.27%(RSD=0.96%)。结论:该方法准确可靠,重现性好,可用于益心酮片的质量控制。

Determination of Five Components in Yixintong Tablets by HPLC

Objective：To establish an HPLC method for determining simultaneously vitexin 4＂-O-glucoside, vitexin rhamnoside, vitexin, rutin and hyperoside in Chinese patent medicine Yixintong Tablets. Methods：The HPLC method was carried out on Sepax HP C18 column （4. 6 mm×250 mm, 5μm） at the temperature 32℃ ;the mobile phase consisted of A：CH3CN-THF（20：1 ）, B：0. 5% Formic Acid gradient elution profile was 0 - 10 min, A ： 14% - 17% , B ： 86% - 83% ; 10 - 26 min, A ： 17% - 18%, B ： 83% - 82% ;26 - 28 min, A：18% - 14% B ：82% -86% ;UV detection wavelength was set at 360 nm. The flow rate was 1.0 mL · min-1. Results：The average recoveries were 99. 61% with RSD 0. 64% for vitexin 4＂-O-glucoside ;99.30% with RSD 0. 49% for vitexin rhamnoside ;98.97% with RSD 1.03% for vitexin;99.45% with RSD 0. 85% for rutin;99.27% with RSD 0. 96% for hyperoside. Conclusions：The method is simple and it has the adequate accuracy and selectivity. It can be used to control quality of Yixintong Tablets.