| 小鼠粒-单系祖细胞体外培养的透射电镜观察 |
| |
| 引用本文： | 李夏新,傅洪林,张肇和.小鼠粒-单系祖细胞体外培养的透射电镜观察[J].南方医科大学学报,1988(3). |
| |
| 作者姓名： | 李夏新 傅洪林 张肇和 |
| |
| 作者单位： | 第一军医大学第一附属医院小儿科，第一军医大学第一附属医院小儿科，第一军医大学第一附属医院 指导教师 |
| |
| 摘 要： | 用液体法培养了C_(57)BL/6J小鼠CFU—GM,并对其进行透射电镜观察,发现在2～6天培养时间内各期集落的细胞组成不同,CFU—GM在体外发育过程中细胞核、线粒体、粗面内质网、游离核糖体、高尔基复合体.糖原颗粒及颗粒的发育有特征性变化,与体内的成熟规律有些差异。同时讨论了粒系细胞与单系细胞的区别.
|
| 关 键 词： | 粒-单系祖细胞 细胞培养 超微结构 透射电镜 |
Ultrastructural study of murine CFU-GM in vitro by trasmission electron microscopy |
| |
| Li Xiaxin,et al.Ultrastructural study of murine CFU-GM in vitro by trasmission electron microscopy[J].Journal of Southern Medical University,1988(3). |
| |
| Authors: | Li Xiaxin |
| |
| Abstract: | We cultured the CFU-GM of C57BL/6J mice in liquid medium and observed the ultrastructural changes of the granulocytes and macrophages during the process of maturation with transmission electron microscope, The cell composition of the colonies was different in various stages in the 2 to 6 days of culture. Both granulocytic cells and macrophagocytic cells had characteristical changes in the nucleus, mitochondria, rough endoplasmic reticulum, free ribosome, Golgi complex, glycogen particles and the maturation of granules, which had a little difference as compared to the changes following maturation of granulocytes and macrophages in vivo. The difference between granulocytic and macrophagocytic cells was also discussed. |
| |
| Keywords: | CFU-GM cell culture ultrastructure transmission electron microscopy |
| 本文献已被 CNKI 等数据库收录！ |
|
