| CIC-3氯通道对H2O2诱导的大鼠主动脉平滑肌细胞凋亡的影响 |
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| 引用本文： | 周园,周家国,丘钦英,黎小妍,刘捷,关永源.CIC-3氯通道对H2O2诱导的大鼠主动脉平滑肌细胞凋亡的影响[J].中国药理学通报,2006,22(10):1175-1179. |
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| 作者姓名： | 周园 周家国 丘钦英 黎小妍 刘捷 关永源 |
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| 作者单位： | 中山大学中山医学院药理教研室,广东广州510080 |
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| 基金项目： | 国家自然科学基金资助项目（No 30472021,30500616）;CMB（No 00730）;广东省自然科学基金资助课题（No 5300822） |
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| 摘 要： | 目的研究CIC-3氯通道蛋白过表达对H2O2诱导的大鼠主动脉平滑肌细胞凋亡的影响。方法蛋白免疫印迹法检测CIC-3蛋白表达；形态学方法、DNA琼脂糖电泳、MTT法和流式细胞仪观察和分析H2O2诱导的大鼠主动脉平滑肌细胞形态学改变、DNA断裂、细胞存活率、凋亡率及CIC-3蛋白过表达对其影响。结果CIC-3蛋白过表达减轻H2O2诱导的大鼠主动脉平滑肌细胞形态学改变、DNA断裂及细胞凋亡率，增加细胞存活。结论提示CIC-3氯通道蛋白过表达保护H2O2诱导的大鼠主动脉平滑肌细胞凋亡。
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| 关 键 词： | 氯通道 CIC-3 凋亡 H2O2 |
| 文章编号： | 1001-1978（2006）10-1175-05 |
| 收稿时间： | 2006-05-09 |
| 修稿时间： | 2006-08-15 |
Effects of CIC-3 chloride channel on H2O2 -induced apoptosis in rat aortic vascular smooth muscle cells |
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| ZHOU Yuan, ZHOU Jia-guo, QIU Qin-ying, LI Xiao-yan, LIU Jie, GUAN Yong-yuan.Effects of CIC-3 chloride channel on H2O2 -induced apoptosis in rat aortic vascular smooth muscle cells[J].Chinese Pharmacological Bulletin,2006,22(10):1175-1179. |
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| Authors: | ZHOU Yuan ZHOU Jia-guo QIU Qin-ying LI Xiao-yan LIU Jie GUAN Yong-yuan |
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| Institution: | Dept of Pharmacology, Zhongshan Medical College, Sun Yat - Sen University, Guangzhou 510080, China |
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| Abstract: | Aim To investigate the effects of CIC-3 protein on H2O2-induced apoptosis in rat aortic vascular smooth muscle cells. Methods Western blot was performed to detect the expression of CIC-3 protein. Morphological analysis, DNA agarose gel electrophore- sis, MTT assay and FACS were performed to measure the morphological changes, apoptotic rate in rat aortic DNA ladder, viability and smooth muscle cells. Resuits Overexpression of CIC-3 protein in rat aortic smooth muscle cells prevents the apoptotic events induced by H2O2 including ultrastructural alteration, DNA ladder and the apoptotic cell death. Conclusion CIC-3 overexpression prevents H2O2-induced apoptosis in rat aortic smooth muscle cells. |
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| Keywords: | CIC-3 chloride channel apoptosis H2O2 |
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