Identification of harman in the rat arcuate nucleus |
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Authors: | D. W. Shoemaker J. T. Cummins T. G. Bidder H. G. Boettger M. Evans |
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Affiliation: | (1) Psychiatry Department and Addiction Research Laboratory, Sepulveda Veterans Medical Center, 16111 Plummer Street, 91343 Sepulveda, CA, USA;(2) Department of Psychiatry, University of California, Los Angeles, USA;(3) Jet Propulsion Laboratory, California Institute of Technology, 4800 Oak Grove Drive, 91103 Pasadena, CA, USA |
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Abstract: | Summary This communication describes the presence of 1-methyl--carboline (harman) in the hypophysiotropic area of the hypothalamus which incorporates the arcuate nucleus. Diethyl ether extracts of boratebuffered arcuate homogenates were subjected to silica column chromatography and thin-layer chromatography (TLC). Identification of the -carboline was accomplished by use of fluorescent spectrometry, gas chromatography (GC), mass spectrometry (MS) and combined gas chromatography-mass spectrometry (GC-MS).Presented in a preliminary report at the 10th Annual Meeting of the American Society for Neurochemistry, Charleston, South Carolina, USA, March 1979 |
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Keywords: | /content/l82k1l6m223k0746/xxlarge946.gif" alt=" beta" align=" MIDDLE" BORDER=" 0" >-Carboline Harman Arcuate nucleus Neuroendocrinology |
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