| PCR法从痰液中快速检测结核分枝杆菌 |
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| 引用本文： | 赵锦荣,闫小君,郭晏海,苏成芝.PCR法从痰液中快速检测结核分枝杆菌[J].第四军医大学学报,1999,20(4):307-308. |
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| 作者姓名： | 赵锦荣 闫小君 郭晏海 苏成芝 |
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| 作者单位： | 第四军医大学基因诊断技术研究所,陕西,西安,710033 |
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| 摘 要： | 目的：建立一种从痰液中检测结核分枝杆菌简便，快速且灵敏的方法。方法；用碱液化，水洗和Ｔｒｉｔｏｎ Ｘ－１００热裂解法从痰液中制备结核分枝杆菌ＤＮＡ，进行ＰＣＲ反应，并将该方法同其它几种方法进行比较。结果：该方法同抗酸性染色镜检法有显差异，同酚／氯仿法具有的灵敏度，结论：用该方法从痰液中检测结核分枝杆菌具有灵敏，简便和快速等特点。
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| 关 键 词： | 结核杆菌 聚合酶链反应 |
A rapid method for detecting mycobacterium tuberculosis from sputum samples |
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| ZHAO Jin-Rong,YAN Xiao-Jun,GUO Yan-Hai,SU Cheng-Zhi.A rapid method for detecting mycobacterium tuberculosis from sputum samples[J].Journal of the Fourth Military Medical University,1999,20(4):307-308. |
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| Authors: | ZHAO Jin-Rong YAN Xiao-Jun GUO Yan-Hai SU Cheng-Zhi |
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| Abstract: | AIM: To establish a simple, rapid and sensitive method detecting mycobacterium tuberculosis
from sputum samples. METHODS: ZWe combined alkali liquefaction and water wash with heat
lysis to prepare mycobacterium tuberculosis DNA used as template in PCR. We also compared
this method with several other methods. RESULTS: The PCR results demonstrate that the
method we have established has the similar sensitivity to the phenol/chloroform method.
CONCLUSION: The method we have established is economical, simple, rapid, and sensitive in
detecting mycobacterium tuberculosis from sputum samples. |
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| Keywords: | DNA |
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