高效液相色谱法测定丹参酮滴耳液中4种丹参酮成分含量

 目的 利用梯度洗脱，建立高效液相色谱测定丹参酮滴耳液中二氢丹参酮Ⅰ、隐丹参酮、丹参酮Ⅰ和丹参酮Ⅱ_A含量的方法。方法 采用高效液相色谱法。Intersil C₁₈分析色谱柱（4.6 mm×250 mm,5 μm），流动相为乙腈-10%乙腈梯度洗脱，流速1.6 mL·min^-1,检测波长254 nm。结果 二氢丹参酮Ⅰ、隐丹参酮、丹参酮Ⅰ和丹参酮Ⅱ_A保留时间分别为11.9，19.2，21.3和28.5 min,与各自相邻峰的分离度均大于1.5，理论板数分别为3?310，4 278，3 624和20 677。二氢丹参酮Ⅰ平均回收率为101.3%，RSD=2.2%，隐丹参酮平均回收率为97.2%，RSD=2.2%，丹参酮Ⅰ平均回收率为102.1%，RSD=3.0%，丹参酮ⅡA平均回收率为99.5%，RSD=1.9%。二氢丹参酮Ⅰ、隐丹参酮、丹参酮Ⅰ和丹参酮Ⅱ_A最低检出浓度分别约为0.6，0.9，0.6和0.3 μg·mL^-1。结论 本法操作简便,测定结果准确可靠，可用于丹参酮滴耳液中二氢丹参酮Ⅰ、隐丹参酮、丹参酮Ⅰ和丹参酮Ⅱ_A的含量测定。

Determination of dihydrotanshinone Ⅰ,cryptotanshinone,tanshinone Ⅰ and tanshinone Ⅱ A in tanshinone ear drops by HPLC

OBJECTIVE A quantitative method was developed for the determination of dihydrotanshinone Ⅰ, cryptotanshinone, tanshinone Ⅰ and tanshinone Ⅱ_A in tanshinone ear drops by HPLC METHODS Column Intersil C₁₈ was used. The mobile phase was composed of acetonitrile - 10% acetonitrile with gradient eluation. The flow rate was 1.6 mL·min^-1 with the wavelength at 254 nm RESULTS The number of theoretical plates calculated by dihydrotanshinone Ⅰ, cryptotanshinone, tanshinoneⅠand tanshinoneⅡ_A peak were 3 310, 4?278, 3 624 and 20 677 respectively. The average recoveries of dihydrotanshinone Ⅰ, cryptotanshinone, tanshinoneⅠ, tanshinone Ⅱ_A were 101.3% (RSD=2.2%), 97.2% (RSD=2.2%), 102.1% (RSD=3.0%) and 99.5% (RSD=1.9%) respectively. The detection limits of dihydrotanshinone Ⅰ, cryptotanshinone, tanshinone Ⅰ and tanshinone Ⅱ_A were about 0.6, 0.9， 0.6 and 0.3 μg·mL^-1 respectively CONCLUSION The method was sensitive, rapid and accurate.