谷氨酰胺对重症急性胰腺炎大鼠腹腔器官血流的影响

目的:研究谷氨酰胺(Gln)对重症急性胰腺炎(SAP)胰腺血流的影响,并探讨其作用机制.方法:将40只大鼠随机分为Gln组(n=16)、SAP组(n=16)和对照组(n=8),术后12和24h处死.用高效液相色谱仪测定血浆Gln浓度变化;用多普勒超声诊断仪测定胰腺局部动脉(胰头和胰体交界处)、肝门静脉、脾动脉和肠系膜上动脉的血流.结果:SAP时,血浆Gln浓度明显下降,经肠道补充Gln能明显提高血浆Gln浓度.与对照组比,SAP大鼠胰腺局部血流术后12 h已有明显下降(P＜0.01).随SAP病程的进展,24h胰腺局部血流下降更严重,与此同时,肝门静脉、脾动脉和肠系膜上动脉的血流量也下降(P＜0.01).与SAP组比,应用Gln能改善胰腺局部以及肝门静脉、脾动脉和肠系膜上动脉的血流量(P＜0.01).结论:Gln能促进SAP大鼠胰腺血流量增加.

The effects of glutamine on the splanchnic blood flow in rats with SAP

Objectives: To investigate the role of glutamine on splanchnic blood flow in SAP rats and underling mechanism. Methods: 32 rats were randomized divided into 2 groups:glutamine group(Gln group, n =16) and SAP group ( n =16).Jejunotomy was performed. Gln group received glutamine.SAP group received saline.All groups were subdivided into 2 groups.8 normal rats were used as the controls.At 12,24hours after the induction of SAP,8 rats in each group were sacrificed.Plasma glutamine concentrations were measured by HPLC.The regional pancreatic microvascular blood flow was measured by Dopplar ultrasound,the blood flow of portal vein,spleen artery and superior mesenteric artery was also measured. Results: Enterally administered Gln was well tolerated by the rats.There was an increase in plasma Gln levels after Gln supplementation on Gln group.The regional pancreatic microvascular blood flow decreased significantly in SAP group( P <0.01),and continued to decrease after 24 h ( vs 12 h, P < 0.01).The blood flow of portal vein,spleen artery and superior mesenteric artery also decreased in SAP group.Glutamine can increase the regional pancreatic microvascular blood flow,meanwhile increase the blood flow of portal vein,spleen artery and superior mesenteric artery( vs SAP group, P < 0.01 ). Conclusions: Enteral administration of glutamine can increase the splanchnic blood flow in SAP rats.