生物素探针在疟疾基因监测中的应用研究

本文报道了用光敏生物素标记恶性疟原虫特异克隆 p BF2 DNA片断作探针 ,以斑点杂交试验检测不同疟区疟疾病人血样和蚊体内的疟原虫。探针检测蚊媒时 ,在 2 0只蚊虫中有 1只感染蚊虫即可被检出 ,也可将单个蚊虫直接压在硝酸纤维素膜上进行检测 ;探针检测血样亦取得良好结果 ,与镜检的符合率 ,恶性疟 96 .6 % ,正常人对照 99.7% ,检测的敏感度为 90个原虫 /μl血。表明该探针在疟防后期的监测中具有较好的实用性

APPLIED STUDIES OF BIOTIN LABELED DNA PROBE IN GENETIC SURVEILLANCE OF MALARIA

Dot blot hybridization with cloned specific pBF2 DNA of Plasmodium falciparum,P.f labeled with photo-biotin as a probe was used to detect the P.f in the blood of patients from different malaria endemic areas and that in mosquitoes.As a result,this probe could detect out 1 infected mosquito when it was mixed with 19 uninfected mosquitoes or single mosquito crushed on NC filter.In the examination P.f patient's blood samples,the probe,based assay had a coincidence rate of 96.6% with microscopic detection and the sensitivity of detection was 90 protozoa/μl blood,while the negative coincidence rate was 99.7% in examining normal blood samlples,showing that this biotin probe has a good applicability in malaria surveillance at late stage of malaria control.