明日叶中查尔酮诱导人胃癌细胞MGC-803凋亡的研究 |
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引用本文: | 王亦敏,马场贵美江.明日叶中查尔酮诱导人胃癌细胞MGC-803凋亡的研究[J].中医临床研究,2011,3(5):67-68. |
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作者姓名: | 王亦敏 马场贵美江 |
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作者单位: | 1. 海南兰地高新科技有限公司,海南,海口,570216 2. 日本大阪药科大学,日本,大阪,101-8301 |
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摘 要: | 目的:研究不同查耳酮成分对人肝癌细胞SMMC-7721增殖的抑制作用。方法:以人胃癌细胞株MGC-803为研究对象,通过MTT法观察不同时间点明日叶中查尔酮成分对细胞增殖的影响,经Hoechst33258和AO/EB染色,再通过荧光显微镜观察其细胞核形态变化。结果:MTT实验结果显示化合物1、2、6、7、9和10,对人肝癌细胞SMMC-7721均有较强的抑制作用,并且显示出明显的剂量依赖关系。随药物浓度的增加,细胞增殖抑制作用明显加强。结论:明日叶中查耳酮类成分有抑制SMMC-7721细胞增殖的作用。
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关 键 词: | 明日叶 查耳酮 4-羟基德里辛(4HD) 黄腐醇(XA) MTT 细胞凋亡 |
A study on inducing human gastric cancer cells MGC-803 with chalcone in tomorrow leaves |
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Abstract: | Objective: To research the inhibitionon of different chalcone ingredients on human liver cancer cell proliferation of SMMC-7721. Methods: Set human cancer cell lines MGC-803 as the research object, observe the influence of tomorrow chalcone leaves components to cell proliferation at different time points by MTT method, initially stained by Hoechst33258 and AO/EB, then Then observed the morphological changes by fluorescence microscopy. Results: MTT results showed that compounds 1, 2, 6, 7, 9 and 10, they all have inhibitory action on human hepatoma cells SMMC-7721, and shows a clear dose-dependent. With the increase of drug concentration, cell proliferation was significantly strengthened. Conclusion: Tomorrow chalcone leaves have the effect of inhibiting SMMC-7721 cell proliferation. |
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Keywords: | Tomorrow leaves Chalcones 4-hydroxyderricin(4HD) Xanthoanglol(XA) MTT Cell Apoptosis |
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