|
|||||
|
|
| 椰毒假单胞菌酵米面亚种鸟嘌呤和胞嘧啶含量的测定及2种测定方法的比较 | |
| 引用本文： | 焦振泉,刘秀梅,杨瑞馥,郭兆彪,河村好章. 椰毒假单胞菌酵米面亚种鸟嘌呤和胞嘧啶含量的测定及2种测定方法的比较[J]. 卫生研究, 2000, 29(4): 243-245 |
| 作者姓名： | 焦振泉 刘秀梅 杨瑞馥 郭兆彪 河村好章 |
| 作者单位： | 1. 中国预防医学科学院营养与食品卫生研究所,北京,100050 2. 中国军事医学科学院微生物与流行病研究所 3. 日本岐阜大学医学部微生物教室 |
| 基金项目： | 国家自然科学基金!资助项目 (No.395 70 6 19) |
| 摘 要： | 利用复性速率法（Ｔｍ值法）和高效液相色谱法（ＨＰＬＣ法）对生化性状比较相近的椰毒假单胞菌酵米面亚种、唐菖蒲伯克霍尔德低菌和椰毒伯克霍尔德氏菌共７个菌株的鸟嘌呤（Ｇ）和胞嘧啶（Ｃ）摩尔百分含量（Ｇ＋Ｃ）％进行测定，并对这２种方法做了对比研究。结果发现，３个种的７个菌株的（Ｇ＋Ｃ）％比较接近，不能否定这３个种的细菌属于同一个种。另外，ＨＰＬＣ法比Ｔｍ值法的准确性好，但重复性不如后者。 |
| 关 键 词： | 椰毒假单胞菌 酵米面亚种 鸟嘌呤 胞嘧啶 |
Determination of guanine plus cytosine content of Pseudomonas cocovenenans subsp farinofermantans with two methods and the comparative study |
|
| Jiao Zhenquan,Liu Xiumei,Yang Ruifu,Guo Zhaobiao,et al.. Determination of guanine plus cytosine content of Pseudomonas cocovenenans subsp farinofermantans with two methods and the comparative study[J]. Journal of hygiene research, 2000, 29(4): 243-245 | |
| Authors: | Jiao Zhenquan Liu Xiumei Yang Ruifu Guo Zhaobiao et al. |
| Affiliation: | Institute of Nutrition and Food Hygiene, Chinese Academy of Preventive Medicine, Beijing 100050, China. |
| Abstract: | The guanine plus cytosine content(G C) mol% of Pseudomonas cocovenenans subsp farinofermantans,Burkholderia gladioli and Burkholderia cocovenenans,which were similar in biochemical characteristics,were determined with Tm values m ethod and HPL C method,and the com parative study of these two methods was discussed. A negative conclusion could not be made from the results. The three bacteria were the same species.The HPL C method was more accurate than the Tm values method,but the repeatability of the latter was better. |
| Keywords: | Pseudomonas cocovenenans subsp farinofermantans guanine plus cytosine content |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |