S-100 protein immunostaining in cells of dendritic morphology within reactive germinal centers by ABC immunoperoxidase method |
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Authors: | Antonino Carbone Riccardo Manconi Alessandro Poletti Rachele Volpe Leonardo Santi |
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Affiliation: | (1) Division of Pathology, Centro di Riferimento Oncologico, I-33081 Aviano;(2) Istituto Nazionale per la Ricerca sul Cancro, Genova, Italy |
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Abstract: | Summary The dendritic and the interdigitating (IRC) reticulum cells are commonly reported to have different topographical distribution in the human lymphoid tissue and some peculiar cytochemical and immunohistochemical features. These include the detection of S-100 protein by PAP (peroxidase anti-peroxidase) method only in IRC located in the thymus-dependent areas. In 15 reactive lymphoid tissue specimens (11 lymph nodes, 3 tonsils, and 1 adenoid) the presence of S-100 protein was tested by ABC (avidin-biotin complex) immunoperoxidase method. IRC were constantly positive. Other positive cells were located within the follicular germinal centers; these immunostained cells appeared as a striking network composed of dendritic-shaped processes displaying a finely granular positivity for S-100 protein. It is suggested that by using this very sensitive technique, S-100 protein can also be detected in intrafollicular cells of dendritic morphology.This study was in part supported by Grant n.84.00525.44 from Consiglio Nazionale delle Ricerche Progetto Finalizzato Oncologia Roma |
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Keywords: | S-100 protein Lymphoid tissue Avidin biotin peroxidase Dendritic cells Interdigitating cells |
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