胃炎患者血清幽门螺杆菌分类抗体免疫印迹检测的临床意义

目的:研究胃炎患者血清幽门螺杆菌分类抗体免疫印迹检测的临床意义。方法:用14C呼气试验检测幽门螺杆菌感染;用免疫印迹法检测幽门螺杆菌分类抗体。结果:152例临床各型胃炎患者中,140人14C-呼气试验阳性(92.11%)。14C-呼气试验阳性组Hp分类抗体中CagA、VacA双阳性或单项阳性者127人(90.71%);阳性预测值90.7%,阴性预测值9.3%。结论:14C-呼气试验与幽门螺杆菌分类抗体两种方法组合检测,对临床幽门螺杆菌的诊断与治疗有重要作用;免疫印迹法检测幽门螺杆菌分类抗体,有简便、敏感、特异、快速的优点。

Clinical significance of detecting the classified antibodies of helicobacter pylori coming from the gastritis patients by immuno-blot method

Objective:To study the clinical significance of detecting the classified antibodies of helicobacter pylori(Hp) in the serum of gastritis patients by immuno-blot method.Methods:The infection of HP and classified antibodies(CagA and VacA) coming from the gastritis patients were respectively detected by 14C-UBT or immuno-blot method.Results:One hundred and forty of 153 patients with various forms of gastritis were positive in 14C-UBT(92.11%),among them 127 cases,whose classified antibodies(CagA and VacA) wre one or two positive,were found im immuno-blot method(90.71%);The positive predictive value was 90.7%,and the negative predictive value was 9.3%.Conclusion:14C-UBT combined with immuno-blot method is very important for the diagnosis and treatment of the infection of Hp.It is simple,sensitive,special and speedy to detect the classified antibodies of HP by immuno-blot method.