| EHF病毒感染家兔后血清特异性抗体水平的动态观察 |
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| 引用本文： | 林雨霖,高友新.EHF病毒感染家兔后血清特异性抗体水平的动态观察[J].免疫学杂志,1990,6(2):106-108. |
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| 作者姓名： | 林雨霖 高友新 |
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| 作者单位： | 湖北医学院病毒研究所，湖北医学院病毒研究所，湖北医学院病毒研究所，湖北医学院病毒研究所，湖北医学院病毒研究所，湖北医学院病毒研究所 武汉 430071，武汉 430071，武汉 430071，武汉 430071，武汉 430071，武汉 430071 |
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| 摘 要： | 本文报告了流行性出血热病毒(Epidemic haemorrhagic fever virus,EHFV)J10、A9株分别感染实验家兔后,用反向间接ELISA(RIELISA)和间接免疫荧光(IFA)方法,动态观察了家兔特异性IgG抗体水平的变化。发现特异性IgG于感染后第1周开始出现,以后抗体水平稳步上升,于第5—7周便达到最高水平,高滴度的抗体可以维持较长时间。结果还表明;RIELISA检测家兔抗体的敏感性明显优于IFA法。用IFA进行的交叉反应还证实;EHFV-J10、A9两种毒株的抗血清能与不同来源的数株病毒发生特异性结合反应。
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| 关 键 词： | 流行性出血热 特异性抗体 病毒感染 |
Sequential Observation of Antibody Response in Rabbits Infected with Epidemic Haemorrhagic Fever Virus |
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| Lin Yulin et al
Virus Research Instutite,Hubei Medical College,Wuhan.Sequential Observation of Antibody Response in Rabbits Infected with Epidemic Haemorrhagic Fever Virus[J].Immunological Journal,1990,6(2):106-108. |
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| Authors: | Lin Yulin
Virus Research Instutite Hubei Medical College Wuhan |
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| Institution: | Lin Yulin et al
Virus Research Instutite,Hubei Medical College,Wuhan 430071 |
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| Abstract: | Rabbits were inoculated with J10 and A9 strains of epidemic haemorrhage fever virus (EHFV) in order to srudy the specific antibody response during EHFV infection. The results showed that the specific IgG of low titre was detected by the reverse indirect ELISA and the indirect immunofluorescence (RIELISA and IFA) one week post-infection. The peak of IgG titres were then detected 6-7weeks post-infection and the high level of antibody was maintained for at least 13 weeks. The antiserum of the rabbits anti-EHFV J10 and A9 strains cross react with a number of strains of EHFV. The results of the experiment also indicated that RIELISA method is more sensitive in detecting the specific anibody than IFA. |
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| Keywords: | Epidemic haemorrhagic fever virus Rabbits specific IgG RIELISA IFA |
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