| 空肠弯曲菌Penner O:19 galE基因真核表达质粒的构建 |
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| 引用本文： | 邢丛丛,李震中,白欣立,徐飞,付金生,马海阳,李文倩,卢小卫. 空肠弯曲菌Penner O:19 galE基因真核表达质粒的构建[J]. 脑与神经疾病杂志, 2012, 20(2): 89-91 |
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| 作者姓名： | 邢丛丛 李震中 白欣立 徐飞 付金生 马海阳 李文倩 卢小卫 |
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| 作者单位： | 1. 050000石家庄,河北医科大学第二医院,河北省神经病学重点实验室,河北省心脑血管病研究所
2. 河北医科大学第二医院儿科,石家庄,050000 |
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| 基金项目： | 国家自然科学基金资助项目，国家高技术研究发晨计划 |
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| 摘 要： | 目的构建空肠弯曲菌Penner O:19 galE基因真核表达质粒。方法 PCR扩增空肠弯曲菌Penner O:19 galE基因,构建pGEM-T-galE克隆质粒及构建pEGFP-N1-galE真核表达质粒,并检测真核表达质粒在293T细胞中的表达。结果构建了真核表达质粒pEGFP-N1-galE,检测到其在293T细胞表达。结论 pEGFP-N1可作为Penner O:19 galE基因的真核表达载体。
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| 关 键 词： | 空肠弯曲菌 galE基因 重组质粒 Penner O:19 |
Construction of eukaryotic expression plasmid of galE gene of campylobacter jejuni Penner O: 19 |
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| XING Cong-cong , LI Zhen-zhong , BAI Xin-li , XU Fei , FU Jin-sheng , MA Hai-yang , LI Wen-qian , LU Xiao-wei. Construction of eukaryotic expression plasmid of galE gene of campylobacter jejuni Penner O: 19[J]. Journal of Brain and Nervous Diseases, 2012, 20(2): 89-91 |
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| Authors: | XING Cong-cong LI Zhen-zhong BAI Xin-li XU Fei FU Jin-sheng MA Hai-yang LI Wen-qian LU Xiao-wei |
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| Affiliation: | .Department of Neurology,the Second Hospital of Hebei Medical University,Shijazhuang 050000,China |
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| Abstract: | Objective Construct eukaryotie expression recombinant plasmid of galE gene of campylobacter jejuni.Methods Cloned the gene galE of C.jejuni Penner O:19,constructed the pGEM-T-galE plasmid and pEGFP-N1-galE eularyotic expression recombinant plasmid,the expression of pEGFP-N1-galE was investigated by transfected into cell 293T.Result pEGFP-N1-galE eukaryotic expression plasmid was constructed successfully and expressed in 293T cell.Conclusion pEGFPN1 eukaryotic expression plasmid is a vector of galE. |
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| Keywords: | Campylobacter jejuni GalE gene Recombinant plasmid Penner O |
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