Simplified urinary oxalate determination using an enzyme electrode |
| |
| Authors: | P Vadgama W Sheldon J M Guy A K Covington M F Laker |
| |
| Affiliation: | 1. Department of Clinical Biochemistry and Metabolic Medicine, Royal Victoria Infirmary, Newcastle upon Tyne NE1 4LP, UK;2. Department of Physical Chemistry, University of Newcastle upon Tyne, Newcastle upon Tyne NE1 7RU UK |
| |
| Abstract: | An enzyme electrode for urine oxalate measurement has been produced using acrylamide gel-entrapped oxalate decarboxylase retained over a CO2 sensor. Urine required pre-treatment with EDTA, but oxalate extraction was not necessary and inhibition by phosphate and sulphate was not apparent until after 10-14 days. The linear range was 0-0.2 mmol/l. Analysis of 50 urine specimens diluted 1 in 10 in pH 3.0 glycine buffer showed good correlation with a widely-used colorimetric method (y = 1.101x-0.018, r = 0.955). |
| |
| Keywords: | To whom correspondence should be addressed. |
| 本文献已被 ScienceDirect PubMed 等数据库收录！ |
