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HPLC法测定鹿蹄草中槲皮素的含量
引用本文:张文,黎钰铃,封丽彬,范卫锋. HPLC法测定鹿蹄草中槲皮素的含量[J]. 中国中医药信息杂志, 2009, 16(9): 47-48
作者姓名:张文  黎钰铃  封丽彬  范卫锋
作者单位:广西玉林制药有限责任公司,广西,玉林,537001
摘    要:目的建立HPLC法测定鹿蹄草中槲皮素含量的方法。方法采用HPLC法,色谱柱:hgilent Eclipse XDB—C18柱(4.6mm×150mm,5μm);流动相:甲醇-0.1%磷酸(50:50);检测波长:370nm。结果槲皮素在0.092~0.46Pg范围内线性关系良好,平均回收率为99.24%,RSD=0.70%。结论方法简便、准确、灵敏度高、重复性好,能有效测定鹿蹄草药材中槲皮素含量。

关 键 词:鹿蹄草  槲皮素  高效液相色谱法  含量测定

Content Determination of Quercetin in Pyrola cailiatha H.Andres by HPLC
Affiliation:ZHANG Wen, LI Yu-ling, FENG Li-bin, et al (Guangxi Yulin Pharmaceutical Co.Ltd., Yulin 537001, China)
Abstract:Objective To set up a method for determining the quercetin in Pyrola calliatha H. Andres. Method HPLC method was carried on Agilent Eclipse XDB-C18 column (4.6 mm× 150 mm, 5 μm) with mobile phase of methanol-0.1% phosphoric acid (50 : 50), and the detecting wavelength was at 370 nm. Result The standard curve was linear within the range of 0.092-0.46 μg. The average recovery rate was 99.24% and RSD was 0.70%. Conclusion The method is simple, accurate, highly sensitive and reproducible. It can be used for the quality determination of quercetin in Pyrola calliatha H. Andres.
Keywords:Pyrola calliatha H.Andres  quercetin  HPLC  content determination
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