p14ARF和p53在子宫内膜癌组织中的表达及其临床意义

目的研究子宫内膜癌组织中p14ARF和p53的表达及意义.方法采用免疫组化LSAB法,检测118例子宫内膜癌及30例正常宫内膜组织中p14ARF及p53的表达.结果p14ARF阴性、弱阳性、中度阳性和强阳性表达率在子宫内膜癌中分别为25.4%(30/118)、38.1%(45/118)、14.4%(17/118)和22.0%(26/118),与正常内膜0(0/30)、56.7%(17/30)、33.3%(10/30)和10.0%(3/30)相比差异有统计学意义,P<0.001,子宫内膜癌中p14ARF表达缺失的比例高于正常宫内膜;p14ARF表达水平与手术病理分期、宫颈间质受累和淋巴结转移相关;与病理分级、组织学类型和肌层侵犯深度无关;不同p14ARF表达水平患者生存率差异无统计学意义,P=0.578 1.p53阴性、弱阳性、中度阳性和强阳性表达率在子宫内膜癌中分别为13.6%(16/118)、40.1%(48/118)、28.0%(33/118)和17.8%(21/118);与正常内膜相比分别为36.7%(11/30)、36.7%(11/30)、16.2%(5/30)和10.0%(3/30)差异有统计学意义,P=0.01,子宫内膜癌中p53阳性表达的比例高于正常宫内膜;p53表达与肿瘤分期、盆腔淋巴结转移和肌层侵犯深度相关,与病理分级、组织学类型和宫颈间质受累无关;不同p53表达水平患者生存率差异无统计学意义,P=0.416 6;在子宫内膜癌组织中p14ARF与p53表达呈负相关,r＝-0.243,P＝0.008.结论p14ARF和p53异常表达与子宫内膜癌发生密切相关,但对其预后的影响有待进一步的研究.

Expressions and clinical significance of p14ARF and p53 in endometrial carcinoma

OBJECTIVE:To study the expressions of p14ARF and p53 in endometrial carcinoma(EC) and the clinical significance. METHODS: The expressions of p14ARF and p53 in EC were detected ty using immunohistochemistry (SLAB method). RESULTS: The negative, weakly positive, moderately positive and strongly positive expressions of p14ARF in EC were 25.4%(30/118),38.1%(45/118),14.4%(17/118) and 22.0%(26/118) respectively, which were significantly different from those in normal endometrium 0(0/30),56.7%(17/30),33.3%(10/30) and 10.0%(3/30) respectively,P<0.001. The expression deletion ratio in EC was higher than that in normal endometrium. The expression of p14ARF was related to surgical stage, cervical stroma involved and pelvic lymph node metastasis, but was not related to the grade, histological type(endometroid, non-endometroid) and myometrial invasion. There was no significantly difference in survival rate of cases with different expressions of p14ARF,P=0.5781. The negative, weekly positive, moderately positive and strongly positive expressions of p53 in EC were 13.6%(16/118),40.1%(48/118),28.0%(33/118) and 17.8%(21/118) respectively which were significantly different from those in normal endometrium 36.7%(11/30),36.7%(11/30),16.2%(5/30) and 10.0%(3/30) respectively) ,P=0.01. The ratio of p53 positive expression in EC was higher than that in normal endometrium. The expression of p53 was related to the surgical stage, pelvic lymph node metastasis and myometrial invasion, but was not related to the grade, histological type (endometroid, non-endometroid) and cervical stroma involved. There was no significantly differentce in survival rate of cases with different expressions of p53,P=0.416 6. The expressions of p14ARF and p53 were negatively correlated, r=-0.243, P=0.008. CONCLUSION: The abnormal expressions of p14ARF and p53 are closely correlated with the progression of EC, but their affect in prognosis demands more study.