人类免疫缺陷病毒抗体初筛试验阳性影响因素分析

目的通过对人类免疫缺陷病毒(HIV)抗体初筛试验阳性的结果分析,提高实验室HIV抗体的检测水平。方法对42例初筛试验阳性的标本用原有试剂加另外一种不同原理或不同厂家的筛查试剂重复检测。如两种试剂复测均呈阴性反应,则报HIV抗体为阴性;如均呈阳性反应,或一阳性一阴性,则报HIV抗体待复查,需送江苏省艾滋病检测确证实验室确认。结果 42例初筛阳性经2种试剂复检有5例均呈阴性反应,11例均呈阳性反应,26例为一阴性一阳性,送江苏省艾滋病检测确证实验室确认阳性仅2例,有较高的假阳性率。结论 HIV抗体初筛阳性的影响因素是试剂的敏感性和特异性以及实验室质量控制的管理。

Analysis of the influence factors of the positive results of HIV antibody screening test

Objective To improve the level of laboratory detection of Anti-HIV through the analysis of the positve results of HIV antibody screening test.Methods The screening test of 42 patients with positive screening test reagent plus the original sample of a different principle or from different manufacturers were conducted repeatly.If two agents were negative,they would be reported as the HIV antibody negative.If they were positive or only one positive and one negative,the HIV antibody of which should be reviewed and be confirmed by the AIDS detectioning laboratory in Jiangsu Province.Results 42 patients with positive resuts of screening test were examined again by the two agents.As a results.There were 5 negative cases and 11 positive cases,and 26 cases were about one positive and one negative.However,only 2 cases were positive after confirmed by AIDS detection laboratory of Jiangsu Province.So there was a higher proportion of false positive.Conclusion The sensitivity of the test reagent and the specificity of laboratory quality control and management are the influence factors of the positive resats of HIV antibody screening test.