| 多发性硬化患者用药前后脑脊液蛋白质组学液相色谱技术体系的建立 |
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| 引用本文： | 王静颖,李芸,刘师莲.多发性硬化患者用药前后脑脊液蛋白质组学液相色谱技术体系的建立[J].山东大学学报(医学版),2010,48(1):85. |
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| 作者姓名： | 王静颖 李芸 刘师莲 |
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| 作者单位： | 1. 聊城职业技术学院遗传学教研室,山东聊城,252000
2. 山东大学医学院生物化学与分子生物学研究所,济南,250012 |
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| 摘 要： | 目的 建立多发性硬化(MS)患者用药前后脑脊液蛋白质组一维、二维液相色谱分离的技术体系.方法 以MS患者用药前后脑脊液为研究对象,提取脑脊液蛋白质组,分别采用超高压液相色谱-四级杆-飞行时间质谱(UPLC-Q-TOF MS)技术和二维色谱聚焦-反向液相色谱(PF-2D)技术进行分离鉴定.结果 分别获得MS患者用药前后脑脊液蛋白质组一维和二维液相色谱图谱,分离方法有良好的重现性.筛选并鉴定出表达有明显差异的蛋白质.结论 用液相色谱技术筛选差异蛋白有助于寻找具有临床治疗意义的MS疾病标识物,为MS疾病的早期诊断和治疗提供理论和实验依据.
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| 关 键 词： | 蛋白质组 色谱法 高压液相 脑脊髓液 多发性硬化 |
| 收稿时间： | 2009-06-23 |
Establishment of liquid chromatography technology platform of cerebrospinal fluid for patients with multiple sclerosis in pre-and post-medication |
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| WANG Jing-ying,LI Yun,LIU Shi-lian.Establishment of liquid chromatography technology platform of cerebrospinal fluid for patients with multiple sclerosis in pre-and post-medication[J].Journal of Shandong University:Health Sciences,2010,48(1):85. |
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| Authors: | WANG Jing-ying LI Yun LIU Shi-lian |
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| Institution: | WANG Jing-ying1,LI Yun2,LIU Shi-lian2(1. Institute of Genetics,Liaocheng Vocational , Technical College,Liaocheng 252000,Sh,ong,China,2. Institute of Biochemistry , Molecular Biology,School of Medicine,Sh,ong University,Jinan 250012,China) |
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| Abstract: | Objective To establish a comparative proteomics technology platform of cerebrospinal fluid (CSF) for patients with multiple sclerosis (MS) in pre-and post-medication by 1D and 2D liquid phase separation methods. Methods Peptides and proteins in CSF proteomics of MS patients in pre-and post-medication were separated by UPLC-Q-TOF MS (ultra-performance liquid chromatography coupled to quadrupole,time-of-flight tandem mass spectrometer) and PF-2D (two-dimensional liquid chromatography). Results Different pepti... |
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| Keywords: | Proteome Chromatography high pressure liquid Cerebrospinal fluid Multiple selerosis |
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