己酮可可碱对大鼠脑缺血再灌注损伤细胞凋亡及Bcl-2、Caspase-3表达的影响

目的：探讨己酮可可碱对大鼠局灶性脑缺血再灌注损伤Bcl-2、Caspase-3表达的影响。方法：sD大鼠随机分为假手术组、脑缺血-再灌注模型组、川芎嗪组和己酮可可碱组，每组10只。术前实验性灌胃给药，川芎嗪给药剂量为50mg／kg，fyllX组给药剂量为100mg／kg，每日-次，连续给药10天。采用线栓法制作大鼠脑缺血-再灌注损伤模型。造模成功2h后再尾静脉注射1次。假手术组和对照组给予生理盐水。用TUNEI．法检测脑缺血2h再灌注24h大鼠皮质和海马神经细胞凋亡率，免疫组化法观察Bcl-2和Caspase-3蛋白表达。结果：与脑缺血-再灌注组比较，川芎嗪组和PTX组皮质和海马部神经细胞凋亡率明显减少（P均〈0．01），Bcl-2表达明显增加（P均〈0．01），Caspase-3表达明显降低（P均〈0．01），川芎嗪组和Prx组两组间比较差异无统计学意义（P〉0．05）。结论：PTX可抑制大鼠神经细胞凋亡，促进Bcl-2表达，降低Caspase-3表达。

Effects of Pentoxifylline on Cell Apotosis and Expression of Bcl-2,and Caspase-3 Protein in Focal Cerebral Ischemia-reperfusion

Objective:To investigate the effect of pentoxifylline on Bcl-2,Caspase-3 expression on focal cerebral ischemia-reperfusion injury rats.Methods:SD rats were randomly divided into four groups:sham operation group,cerebral ischemia-reperfusion model group and TMP group and the pentoxifylline group.10 in each group.The rats with drugs treatment were undertaken middle cerebral artery occlusion(MCAO)and reperfution after administrating drugs consecutively for 10 days via intragastric administration and administra...