应用环磷酰胺构建C57BL/6J小鼠免疫抑制模型

背景：在免疫增强剂的研究中,常用到免疫抑制模型,如何建立免疫抑制模型成为免疫增强剂作用评价的关键。目的：应用环磷酰胺构建C57BL/6J小鼠免疫抑制模型。方法：将小鼠随机分为正常对照组、环磷酰胺50mg/kg5d组,环磷酰胺80mg/kg3d组,环磷酰胺80mg/kg5d组,环磷酰胺100mg/kg5d组和环磷酰胺100mg/kg隔天给药组。正常对照组小鼠腹腔注射生理盐水0.1mL,连续5d。环磷酰胺50mg/kg5d组,环磷酰胺80mg/kg3d组,环磷酰胺80mg/kg5d组和环磷酰胺100mg/kg5d组小鼠分别以环磷酰胺50,80,80,100mg/kg腹腔注射连续5,3,5,5d。环磷酰胺100mg/kg隔天给药组小鼠腹腔注射100mg/kg环磷酰胺,隔天1次,共注射3次。结果与结论：与正常对照组比较,腹腔注射环磷酰胺可导致小鼠外周血中CD3＋T,CD3＋CD4＋T及CD3＋CD8＋T细胞明显下降（P〈0.05）;谷丙转氨酶（除环磷酰胺50mg/kg5d、80mg/kg3d组）、谷草转氨酶、尿素显著升高（P〈0.05）,其中环磷酰胺80mg/kg5d组、环磷酰胺100mg/kg5d组和环磷酰胺100mg/kg隔天给药组对肝肾功能的影响更为明显。提示腹腔注射环磷酰胺可建立CD3＋T,CD3＋CD4＋T及CD3＋CD8＋T细胞明显下降的免疫抑制模型,其中以环磷酰胺50mg/kg5d和80mg/kg3d方式对肝肾功能的损伤较小。

Establishing a C57BL/6J mouse immunosuppressive model induced by cyclophosphamide

BACKGROUND： The immunosuppressive models are commonly used in the research of immunostimulants, but how to establish the immunosuppressive models is the key for the evaluation of immunostimulants. OBJECTIVE： To establish C57BL/6J mice immunosuppressive model induced by cyclophosphamide METHODS： Normal C57BL/6J mice were randomly divided into six groups （n=4）. Group 1 was normal control group, group 2 was injected with 50 mg/kg cyclophosphamide for 5 days, group 3 was injected with 80 mg/kg cyclophosphamide for 3 days, group 4 was injected with 80 mg/kg cyclophosphamide for 5 days, group 5 was injected with 100 mg/kg cyclophosphamide for 5 days, and group 6 was injected with 100 mg/kg cyclophosphamide every 2 days. Mice in group 1 were intraperitoneal injected with 0.1 mL normal saline and lasted for 5 days. Mice in group 2, 3, 4 5 were intraperitoneal injected with 50, 80, 80 and 100 mg/kg cyclophosphamide, respectively, and lasted for 5, 3, 5 and 5 days, respectively. Mice in group 6 were intraperitoneal injected with 100 mg/kg cyclophosphamide every two days and total injected for three times. RESULTS AND CONCLUSION： Compared with group 1, the CD3＋T, CD3＋CD4＋T and CD3＋CD8＋T levels in the peripheral blood in the other five groups were significantly decreased （P 〈 0.05）; the contents of alanine aminotransferase （except for group 2 and group 3）, aspartate aminotransferase and blood urea nitrogen in the other five groups were significantly increased （P 〈 0.05）, especially in group 4, 5 and 6. Intraperitoneal injection of cyclophosphamide can establish the immunosuppressive model with decreased level of CD3＋T, CD3＋CD4＋T and CD3＋CD8＋T, and intraperitoneal injection of 50 mg/kg and 80 mg/kg cyclophosphamide for 5 and 3 days has less kidney and liver damage.