枸杞多糖对胎儿卵巢组织冷冻保存的影响

背景：抗氧化剂的应用对于提高卵巢组织冻存后的活力起着很重要的作用。目的：通过观察冷冻保存的胎儿卵巢组织异种移植到裸鼠肾被膜下的发育情况,探索枸杞多糖在卵巢组织冷冻保存中的作用。方法：实验分为4组。①新鲜移植组：取材后的新鲜胎儿卵巢组织直接进行移植。②冻存对照组：冷冻保护液为基液。③β-巯基乙醇组：冷冻保护液为基液添加100μmol/Lβ-巯基乙醇。④枸杞多糖组：冷冻保护液为基液添加400mg/L枸杞多糖。对冷冻复温后的胎儿卵巢皮质块进行祼鼠的肾被膜下移植,并于移植后12周取材。结果与结论：各组在移植物的存活率上差异无显著性意义（P〉0.05）。在卵泡计数上冷冻对照组最低（P〈0.05）。在卵泡的存活率上,各组间差异均有显著性意义,其中以冷冻对照组最低,枸杞多糖组最高。β-巯基乙醇组和枸杞多糖组卵巢超微结构较冷冻对照组保存的好。提示400mg/L的枸杞多糖和100μmol/L的β-巯基乙醇有利于卵巢组织的冷冻保存,并可显著提高冷冻卵巢组织移植后的存活率。

Effects of lycium barbarum polysaccharides on fetal ovarian tissue cryopreservation

BACKGROUND： The application of antioxidant plays a key role in improving the viability of frozen-thawed ovarian tissue OBJECTIVE： To explore the effects of lycium barbarum polysaccharides on fetal ovary tissue cryopreservation by observe the developmental condition of cryopreserved fetal ovarian tissue xenografts after transplanted into nude mice kidney capsule. METHODS： The experiment was divided into four groups. ①Fresh transplanted group： the fresh fetal ovary tissue was transplanted directly. ②Cryopreservation control group： the cryoprotectant solution was the base fluid. ③β-mercaptoethanol group： the cryoprotectant solution was the basic fluid with 100 μmol/L β-mercaptoethanol. ④Lycium barbarum polysaccharides group： the cryoprotectant solution was the basic fluid with 400 pmol/L lycium barbarum polysaccharides. The ovarian cortex block was transplanted into the nude mice kidney capsule after freezing thawing, and the specimen was obtained at 12 weeks after transplantation. RESULTS AND CONCLUSION： There was no significant difference of the survival rate in the grafts among groups （P 〉 0.05）. The cryopreservation control group had the most follicle （P 〈 0.05）. As for the survival rate of follicles, there was significant difference among groups; it was lowest in cryopreservation control group and highest in lycium barbarum polysaccharides group. Preservation of ovarian ultrastructure in β-mercaptoethanol and lycium barbarum polysaccharides group was better than that in the cryopreservation control group. Lycium barbarum polysaccharides with concentration of 400 mg/L and β-mercaptoethanol with concentration of 100 mg/L is benefit to the ovarian tissue cryopreservation and can significantly improve the survival ratio of cryopreserved ovarian tissue after transplantation.