大黄酸可影响高糖培养SD大鼠肾小球系膜细胞的凋亡

背景：含有大黄的复方中药消可宁能有效防治早期糖尿病肾病。目的：观察大黄酸对髙糖培养的SD大鼠肾小球系膜细胞凋亡的影响。方法：以20,40,80μmol/L浓度的大黄酸刺激髙糖培养的肾小球系膜细胞,苏木精-伊红染色观察凋亡细胞形态,DAPI荧光染色观察细胞核凋亡情况,流式细胞仪观察细胞凋亡率。结果与结论：苏木精-伊红染色及DAPI染色结果显示大黄酸可促进髙糖培养的肾小球系膜细胞的凋亡,且与浓度与时间成正相关。各组肾小球系膜细胞的细胞凋亡率差异有显著性意义（P〈0.05）,表明大黄酸对髙糖培养的肾小球系膜细胞凋亡产生影响且与浓度及时间成正相关。

Rhein effects on apoptosis of glomerular mesangial cells cultured with high glucose in Sprague Dawley rats

BACKGROUND： Our previous study has already manifested that Chinese medicine Xiaokening containing Rhein can effectively control the development of early diabetic nephropathy. OBJECTIVE： To investigate the effect of Rhein on apoptosis of glomerular mesangial cells （GMCs） of Sprague Dawley rats cultured with high glucose. METHODS： The GMCs cultured with high glucose were stimulated by different concentrations of Rhein （20, 40, 80 μmol/L）. Morphology of GMCs was discriminated by hematoxylin-eosin staining. Karyon apoptosis was examined by fluorescence staining of 4＇,6-diamidino-2-phenylindole （DAPI）. Cell apoptosis rate was detected by flow cytometry. RESULTS AND CONCLUSION： The outcome of hematoxylin-eosin staining and 4＇,6-diamidino-2-phenylindole staining indicated that Rhein could obviously induce apoptosis of GMCs in a dose- and time-dependent manner. The apoptosis rate had significant difference among different groups （P 〈 0.05）. This result shows that Rhein can obviously induce apoptosis of GMCs cultured with high glucose and in a dose- and time-dependent manner.