Development of a duplex PCR assay for detection of Brachyspira hyodysenteriae and Brachyspira pilosicoli in pig feces |
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| Authors: | La Tom Phillips Nyree D Hampson David J |
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| Institution: | School of Veterinary and Biomedical Sciences, Murdoch University, Murdoch, Western Australia 6150, Australia. |
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| Abstract: | A duplex PCR (D-PCR) amplifying portions of the Brachyspira hyodysenteriae NADH oxidase gene and the B. pilosicoli 16S rRNA gene was developed and then tested on DNA extracted from 178 porcine fecal samples. The feces also underwent anaerobic culture and species-specific PCRs. Fecal extraction-D-PCR detected seven additional samples containing B. hyodysenteriae and five more containing B. pilosicoli. |
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