Catecholamines in the yolk sac epithelium of the rat |
| |
Authors: | Margret Schlumpf and Walter Lichtensteiger |
| |
Institution: | (1) Institute of Pharmacology, University of Zürich, Gloriastrasse 32, CH-8006 Zürich, Switzerland |
| |
Abstract: | Summary Catecholamines were found histochemically in the visceral yolk sac of the rat from embryonic day (ED) 10, i.e. before the amines become detectable in peripheral or central neurons of the fetus. Formaldehydeinduced fluorescence was confined to the apical part of the yolk sac epithelial cells. The specificity of histofluorescence has been confirmed by borohydride reduction, microspectrofluorimetry revealing an emission peak at 480 nm and administration of reserpine. The catecholamines present were identified by mass fragmentography using N,O-trifluoroacetyl derivatives. At ED 13 both dopamine and norepinephrine were present, while only dopamine was detected at ED 181/2. Maternal circulation or the epithelial cells themselves appear as possible sources of these catecholamines. The occurrence of amines in the yolk sac epithelium may reflect an intracellular role of these compounds, a barrier function of the epithelium or a step in a transport to the fetus where the amines might assume regulatory functions.Supported by grants from SNSF 3.231-0.77, Hartmann Müller Stiftung and Bruner FoundationPreliminary reports were presented at the Fourth International Catecholamine Symposium, Pacific Grove, USA, 1978 (Schlumpf et al., 1979), and at the International Symposium on Development and Chemical Specificity of Neurons , Schatzalp, Davos, 1978 |
| |
Keywords: | Yolk sac Prenatal catecholamines Extraneuronal catecholamine localization Rat |
本文献已被 SpringerLink 等数据库收录! |
|