维生素K1注射乳在小鼠体内的药物分布评价

目的 制备维生素K₁注射乳,并考察其在小鼠体内的分布情况。方法 采用微射流仪制备维生素K₁注射乳;维生素K₁注射乳剂和普通注射剂尾静脉注射至小鼠体内,采用HPLC法测定维生素K₁在小鼠体内各组织的浓度。结果 维生素K₁注射乳粒径为（172.2±63.0）nm,注射给药后,维生素K₁注射乳组的肝、脾中维生素K₁的AUC值分别为注射液组的2.34和2.70倍。结论 维生素K₁注射乳在小鼠体内具有显著肝、脾靶向性

In vivo distribution of vitamin K1 emulsion for injection in mice

Objective To prepare vitamin K₁ emulsion for injection, and evaluate its in vivo distribution in mice. Methods Vitamin K₁ emulsion for injection was prepared by microfluidizer treatment. The vitamin K₁ emulsion and conventional vitamin K₁ injection were administered into mice through tail-vein, and the drug concentrations in various tissues were determined by HPLC method. Results The resulted emulsion showed a mean diameter of (172.2±63.0) nm. After the administration of emulsion formulation, the AUC values of vitamin K₁ in liver and spleen were 2.34 and 2.70 times higher than conventional vitamin K₁ injection, respectively. Conclusions The results show that vitamin K₁ emulsion possessed better targeting ability to liver and spleen.