p27和p-ERK1/2在肝细胞癌中的表达及相关性研究

目的 探讨p27和p-ERK1/2在肝细胞癌(hepatocellular carcinoma,HCC)中的表达、意义及二者间可能的关系.方法 免疫组织化学(S-P)法检测肝癌50例、肝硬化15例及正常肝组织10例中p27和p-ERK1/2的表达.结果 肝癌组织中p27的阳性表达率(42%)明显低于肝硬化(78%)和正常肝组织(90%),表达水平与肿瘤恶性程度、大小、是否广泛转移等有关(P＜0.05);肝癌组织中p-ERK1/2的阳性表达率(36%)显著高于肝硬化(21.5%)和正常肝组织(11.6%),表达与恶性程度、转移等有关;肝癌组织中p27的表达与p-ERK1/2表达呈负相关(rs=-0.4812,P＜0.01).结论 p-ERK1/2基因的激活可以影响p27的表达,二者与肝癌的发生、发展有关.

Expression and relationship of p27 and p-ERK1/2 proteins in hepatocellular carcinoma

Objective To investigate the expression and signifinance of p27 and p-ERK1/2 proteins in human hepatocellular carcinoma(HCC),and the possible relationship between them.MethodsThe expression of p27 and p-ERK1/2 proteins was detected in 50 HCC tissues,15 liver cirrhosis tissues and 10 normal liver tissues by immunohistochemistry.ResultsThe positive expression rate of p27 in HCC tissues was 42%,significantly lower than in liver cirrhosis tissues(78%)and normal liver tissues(90%).The expression levels of p27 was related to the malignancy,tumor size and extensive metastasis(P<0.05).The positive expression rate of p-ERK1/2 in HCC tissues was 36%,which was significantly higher than that in liver cirrhosis tissues(21.5%)and normal tissues(11.6%,P<0.05).The expression level of p-ERK1/2 was related to histological differentiation and metastasis(P<0.05).There is a significant inverse correlation between the expression of p27 and p-ERK1/2(rs=-0.4812,P<0.01).ConclusionThe activity of ERK1/2 can regulate the expression of p27 and both of them may play an important role in the genesis and development of HCC.