负载宫颈癌肿瘤裂解物的树突状细胞诱导的淋巴细胞反应

目的:利用树突状细胞(dendritic cell,DC)递呈肿瘤抗原的生物学特性,观察DC负载宫颈肿瘤裂解物抗原后能否促进同种异体T淋巴细胞增殖,并且活化为细胞毒性T淋巴细胞(cytotoxic T lymphocyte,CTL).方法:宫颈癌患者外周血单核细胞体外经粒-巨噬细胞集落刺激因子(GM-CSF)和白细胞介素-4(IL-4)诱导产生DC,肿瘤裂解物和TNF-α共同促其成熟,与异体T淋巴细胞混合共育,以MTT法测定T细胞的增殖程度.结果:宫颈癌患者外周血来源DC无论负载肿瘤组织裂解物还是传代肿瘤细胞裂解物,均可促进异体T细胞大量增殖,二者间无显著差异(P＜0.05).结论:DC能递呈宫颈癌肿瘤抗原,诱导产生大量抗原特异性CTL,提示负载肿瘤裂解物的DC具有为宫颈癌患者进行特异性免疫治疗的临床应用前景.

Cervical tumor lysate- loaded dendritic cell induce the generation of lymphcyte

Objective:To make use of the characteristics of presenting and processing tumor antigen of dendritic cell to enhance generation of isotypical but xenogenous T lymphocyte.Methods:Autologous dendritic cells were obtained by culturing peripheral blood monocyte of patients with cervical cancer in the presence of granulocyte-macrophage colony stimulating factor(GM-CSF) and interleukin-4(IL-4).Dendritic cells were loaded with tumor cell lysate and co-cultured with heterogenous PBMCs from patients to induce generation of tumor specific cytotoxic T cell.The generation rate was measured by MTT.Results:Tumor lysate-loaded DC which was isolated from peripheral blood monocyte of patients with cervical cancer could induce the generation of isotypical but xenogenous T lymphcyte.There was no significant difference between tumor tissue lysate and tumor cell strain lysate(P<0.05).Conclusion:These results indicate that DC could process and present tumor lysate antigen to stimulate generation of specific immunotherapy in patients with cervical cancer.