大鼠gp130反义寡核苷酸阻断rhIL-6对大鼠急性髓系白血病细胞系R2体外增殖的抑制效应

IL 6受体 (IL 6R) β链是分子量为 130kD的膜结合糖蛋白 (gp130 ) ,它是介导IL 6生物效应的信号转导子。我们的实验已经证实了重组人IL 6 (rhIL 6 )可作用于大鼠急性髓系白血病细胞系R2 ,rhIL 6与R2细胞表达的hIL 6R结合并与大鼠 gp130缔合而转导IL 6的信号 ,产生其生物效应。本文在体外液体培养中试验观察到 ,大鼠 gp130的反义寡核苷酸片段被摄入R2细胞后 ,对rhIL 6生物效应产生明显的影响。我们的研究结果表明 ,所合成的大鼠gp130反义寡核苷酸片段在细胞体外液体培养中可部分阻断rhIL 6对R2细胞的增殖抑制效应 ,在最适的终浓度 ,其阻断率可达 (45± 7) %。

Antisense Oligonucleotide of Rat gp130 Blocked the Inhibitory Effect of rhIL-6 on Proliferation of the Rat Acute Myeloid Leukemia Cell Line R2 In Vitro Cultures

The second nonligand binding chain of IL-6 receptor (IL-6R), the membrane glycoprotein with 130 kD mol wt (gp130), is responsible for the signal transduction of IL-6 biological activity. Our experiments indicated that the rat gp130 molecule, which was expressed in the rat acute myeloid leukemia cell line R2, could associate with the complex of rhIL-6 and membrane human IL-6R molecule and transduce the inhibition-inducing signal on R2 cells. In the present study, antisense oligonucleotides of rat gp130 were synthesized and uptaked into the R2 cells. Then the effects of the antisense or sense nucleic acids of rat gp130 on the inhibition induced by rhIL-6 in the R2 cell were investigated. Our results show that the antisense oligonucleotide of rat gp130 blocked the inhibitory effect of rhIL-6 on the R2 cells by (45 +/- 7)% at the optimal concentration