sPD-1/sPD-L1与Th1/Th2及Th17/Treg相关细胞因子在原发免疫性血小板减少症中的研究

目的:探讨原发免疫性血小板减少症(ITP)中s PD-1/s PD-L1的表达情况及其与Th1/Th2、Th17/Treg的平衡关系。方法:采用酶联免疫吸附试验(ELISA)分别检测35例初诊ITP患者和20例健康体检者外周血血清中s PD-1、s PD-L1、IFN-γ、IL-4、IL-17、TGF-β的水平。结果:①与正常对照组相比,ITP患者s PD-1、IFN-γ、IL-17水平升高(P=0. 008、P=0. 031、P=0. 005),IL-4、TGF-β水平降低(P=0. 028、P=0. 042),s PD-L1水平无明显变化(P=0. 107)。②Pearson相关性分析显示:s PD-1与IFN-γ呈正相关(r=0. 516,P=0. 006),与血小板计数呈负相关(r=-0. 562,P=0. 002);血小板计数与IFN-γ呈负相关(r=-0. 531,P=0. 004); s PD-1与IL-4、IL-17、TGF-β无相关性(P均>0. 05)。结论:s PD-1可能通过抑制PD-1/PD-L1负性调节通路,导致T亚群失衡,在ITP疾病中发挥一定的作用。

Study on sPD-1/s PD-L1 and Th1/Th2 and T17/Treg related cytokine in patients with primary immune thrombocytopenia

Objective:To investigate the expression of sPD-1/sPD-L1 and its balance relationship with Th1/Th2,Th17/Treg in primary immune thrombocytopenia(ITP).Methods:The levels of sPD-1,sPD-L1,IFN-γ,IL-4,IL-17 and TGF-βwere detected in 35 patients with initial diagnosis of ITP and 20 healthy subjects by enzyme-linked immunosorbent assay(ELISA).Results:①Compared with the normal control group,the level of sPD-1,IFN-γand IL-17 in ITP patients increased(P=0.008,P=0.031,P=0.005),and IL-4 and TGF-βwere decreased(P=0.028,P=0.042),and no significant change in sPD-L1 level(P=0.107).②The correlation analysis showed that sPD-1 was positively correlated with IFN-γ(r=0.516,P=0.006),negatively correlated with platelet count(r=-0.562,P=0.002).The platelet count was negatively correlated with IFN-γ(r=-0.531,P=0.004).There was no correlation betweens PD-1 and IL-4,IL-17 and TGF-β(P>0.05).Conclusion:sPD-1 may inhibit the PD-1/PD-L1 negative regulation pathway and lead to the imbalance of T cells subgroups and play an important role in ITP disease.