iNKT细胞对哮喘小鼠肺树突状细胞表面分子和促炎性细胞因子表达水平的影响

目的:观察iNKT细胞对哮喘小鼠肺树突状细胞(LDCs)表面分子和促炎性细胞因子表达水平的影响。方法:24只野生型BALB/c小鼠随机分为正常对照组、哮喘组和过继转移组,每组8只; 12只CD1d^-/--BALB/c小鼠随机分为CD1d^-/-对照组和CD1d^-/-哮喘组,每组6只。哮喘组、过继转移组和CD1d^-/-哮喘组小鼠以卵清白蛋白致敏和激发,正常对照组和CD1d^-/-对照组以等量PBS替代,其中过继转移组在第1次激发前1 h给予iNKT细胞尾静脉注射。采用流式细胞仪检测各组小鼠LDCs及表面分子MHCⅡ、CD80、CD86和CD40的表达水平; ELISA法检测LDCs体外培养上清液IL-12p70、IL-6、TNF-α和IL-10水平。结果:过继转移组小鼠LDCs数量和MHCⅡ、CD40、CD80、CD86表达水平及LDCs体外培养上清液IL-12p70、IL-6、TNF-α水平明显高于哮喘组(P<0. 05或P<0. 01); CD1d^-/-哮喘组小鼠LDCs数量和MHCⅡ、CD40、CD80、CD86表达水平及LDCs体外培养上清液IL-12p70、IL-6、TNF-α水平明显低于哮喘组(P<0. 05或P<0. 01),但明显高于正常对照组和CD1d^-/-对照组(P<0. 05或P<0. 01)。结论:iNKT细胞可以增强哮喘小鼠LDCs表面分子和促炎性细胞因子的表达水平。

Effect of iNKT cells on expression of surface molecules and proinflammatory cytokines of lung dentritic cells in an OVA-induced mouse model of asthma

Objective:To investigate the effect of iNKT cells on the expression of surface molecules and proinflammatory cytokines of lung dentritic cells(LDCs)in an OVA-induced mouse model of asthma.Methods:Twenty-four wild type(WT)BALB/c mice were randomly divided into normal control group(n=8),asthma group(n=8)and adoptive transfer group(n=8).Twelve CD1d-/--BALB/c mice were randomly divided into CD1d-/-control group(n=6)and CD1d-/-asthma group(n=6).The mouse model of asthma was induced through sensitization with intraperitoneal administration of ovalbumin(OVA)and intranasal challenge in asthma group,adoptive transfer group,and CD1d-/-asthma group,whereas in normal control group and CD1d-/-control group where PBS was used instead.Adoptive transfer of iNKT cells in adoptive transfer group and PBS in asthma group were carried out 1 h prior the first challenge.The number of LDCs and expression of MHCⅡ,CD40,CD80,CD86 on LDCs were detected by flow cytometry.Levels of IL-12 p70,IL-6,TNF-αand IL-10 in the culture supernatants of LDCs were measured by ELISA.Results:The number of LDCs and expression of MHCⅡ,CD40,CD80,CD86 on LDCs,as well as the levels of IL-12 p70,IL-6 and TNF-αin culture supernatants of LDCs from adoptive transfer group were markedly increased,compared with asthma group(P<0.05 or P<0.01).Moreover,the number of LDCs,MHCⅡ+LDCs,CD40+LDCs,CD80+LDCs,CD86+LDCs as well as the levels of IL-12 p70,IL-6,TNF-αin culture supernatants of LDCs from CD1d-/-asthma group were significantly decreased compared with asthma group(P<0.05 or P<0.01),but was markedly increased compared with control group and CD1d-/-control group(P<0.05 or P<0.01).Conclusion:iNKT cells can enhance the expression of surface molecules and proinflammatory cytokines of LDCs in an OVA-induced mouse model of asthma.