宫颈鳞癌及宫颈上皮内瘤变组织中PINCH、nm23-H1蛋白的表达及临床意义

目的探讨PINCH蛋白和nm23-H1蛋白在宫颈鳞癌及宫颈上皮内瘤变（CIN）组织中的表达及临床意义。方法应用免疫组化SP法检测60例宫颈鳞癌、30例CIN和20例正常宫颈上皮组织中PINCH和nm23-H1蛋白的表达,并结合临床病例特征进行分析。结果 PINCH蛋白在正常宫颈、CIN和宫颈鳞癌间质中的阳性表达率分别为0.0（0/20）、33.3%（10/30）、70.0%（42/60）,呈现逐渐增高的趋势（P〈0.01）;nm23-H1蛋白的阳性表达率分别为95.0%（19/20）、70.0%（21/30）、46.7%（28/60）,呈现逐渐降低的趋势（P〈0.01）。PINCH和nm23-H1的表达与患者的淋巴结转移及FIGO分期有关（P〈0.01）,与患者年龄、肿瘤的组织病理分级及肿瘤大小无关（P〉0.05）。PINCH和nm23-H1在CIN和宫颈鳞癌中表达均有相关性（C值分别为0.697和0.531,P〈0.01）。结论 PINCH蛋白和nm23-H1蛋白可能协同参与了调控宫颈鳞癌的发生、发展过程,并在宫颈鳞癌的转移和淋巴结转移中起一定作用。

The experssion and clinical significance of PINCH and nm23-H1 protein in cervical squamous cell carcinoma and cervical intraepithelial neoplasia

Objective To investigate the expression and clinical significance of PINCH protein(particularly interesting new cysteine-histidine rich protein) and nm23-H1 protein(nonmetastatic protein 23 homologue 1)in cervical squamous cell carcinoma and cervical intraepithelial neoplasia.Methods The expressions of PINCH protein and nm23-H1 protein were detected by immunohistochemistry(SP) in 60 squamous cell carcinomas,30 cervical intraepithelial neoplasias(CINⅠ~Ⅲ) and 20 normal cervical tissue samples,furthermore,the correlation between the expression of two proteins and the clinicopathological characteristics were analyzed.Results The positive expression rates of PINCH protein were 0(0/20),33.3%(10/30),70.0%(42/60) in normal cervical tissues,cervical intraepithelial neoplasias and squamous cell carcinomas respectively,with a gradual increase trend.However the positive expression rates of nm23-H1 protein were 95.0%(19/20),70.0%(21/30),46.7%(28/60) respectively,with a gradual decrease trend,and there were significant differences among them(P<0.05).The expressions of PINCH and nm23-H1 protein were closely correlated with the patients’ lymph node metastases and FIGO staging(P<0.01),however,which were not related with patients’ age,histopathological staging and tumor size(P>0.05).There were correlations in the expression of two proteins between cervical intraepithelial neoplasias and squamous cell carcinomas(C=0.697 and 0.531 respectively,P<0.01).Conclusion The PINCH and nm23-H1 may be involved in the tumorigenesis and development of cervical cancer cooperatively,which may play a certain role in the metastasis of cervical cancer and lymph node metastasis of tumor.