不同浓度HAES在正常人体外对IL-10和IL-12的影响

目的 探讨不同浓度的羟乙基淀粉类血浆代用品(贺斯6%HAES200/0.5)对正常人体免疫系统白介素-10(IL-10)和白介素-12(IL-12)的影响,以检验其是否抑制正常人体的免疫系统.方法 正常志愿者30名,严格无菌条件下抽取静脉血各20 ml,并以肝素抗凝,在超净台内分离出单个核细胞,计数后将细胞平均分成5组...

Effects of hydroxyethyl starch on IL-10 and IL-12 in healthy subjects

Objective To observe the influence of colloid plasma substitutes(hydroxyethyl starch) with different concentrations on IL-10 and IL-12 in healthy subjects,and to evaluate weather hydroxyethyl starch(HS) could inhibit the immune system of healthy people.Methods 30 healthy volunteers were enrolled in the investigation whose venous blood 20ml was taken out under sterile condition,and the blood samples were anticoagulated by heparin,then the concentrations of IL-10 and IL-12 were detected by ELISA in five groups:1640 culture medium control group,10% plasma group,10% HS group,20% HS group and 40% HS group.Results As compared with those in 1640 culture medium control group and 10% plasma group,although the levels of IL-10 and IL-12 in three trial groups of different concentrations HS had little difference under three conditions(negative control,plus LPS,plus PHA),there were no significant differences among them(P>0.05),however,there were significant differences between the two groups,respectively among negative control groups,LPS groups and PHA groups(P<0.05).Conclusion The hydroxyethyl starch does not inhibit immune system of healthy persons in vitro.