| 豚鼠心肌细胞中Kir2.1对内向整流钾电流组成的贡献 |
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| 引用本文： | 杨慧芳.豚鼠心肌细胞中Kir2.1对内向整流钾电流组成的贡献[J].河北医药,2011,33(13):1943-1944. |
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| 作者姓名： | 杨慧芳 |
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| 作者单位： | 河北省石家庄市中心医院,050011 |
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| 摘 要： | 目的 研究心肌细胞中Kir2.1对心肌细胞静息电位中内向整流电流组成的贡献.方法 采用二型胶原酶急性分离豚鼠心室肌细胞,利用膜片钳技术,研究500 mmol/L Ba2+阻断豚鼠心肌细胞内向整流钾电流Kir2.1,从而确定Kir2.1在所有内向整流电流所占比例.结果 Ba2+对豚鼠心肌细胞内向整流钾电流Kir2.1有较...
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| 关 键 词： | 钾通道 膜片钳术 豚鼠 |
The contribution of Kir2.1 to the inward rectifier K+ currents in the cardiomyocytes of guinea pig |
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| YANG Huifang.The contribution of Kir2.1 to the inward rectifier K+ currents in the cardiomyocytes of guinea pig[J].Hebei Medical Journal,2011,33(13):1943-1944. |
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| Authors: | YANG Huifang |
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| Institution: | YANG Huifang.Shijiazhuang Central Hospital,Shijiazhuang 050011,China |
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| Abstract: | Objective To investigate the contribution of Kir2.1 to the inward rectifier potassium channels in the cardiomyocytes of guinea pig during resting potential.Methods The cardiomyocytes of guinea pig were isolated by collagenase II.The patch clamp technique was used to detect the inhibition of 500 mM Ba2＋ on Kir2.1 in ventricular cardiomyocytes of guinea pig,and to determine the proportion of Kir2.1 in all the inward rectifier potassium channels.Results The Ba2＋ had a strongly inhibitory effect on Kir2.1,and Kir2.1 accounted for（81±7）% of the inward rectifier potassium channels in cardiomyocytes of guinea pig.Conclusion The Kir2.1 accountes for about 80% of the inward rectifier potassium channels in cardiomyocytes of normal guinea pig,which plays an important role in maintaining the normal function of cardiomyocytes. |
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| Keywords: | Kir2 1 patch clamp guinea pig |
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