姜黄素对大鼠缺血性脑损伤炎症反应和血脑屏障通透性的影响

目的研究姜黄素对大鼠脑缺血/再灌注损伤炎症反应和血脑屏障通透性的作用,并进一步探讨其潜在的机制。方法利用线栓法制备大鼠脑缺血/再灌注损伤模型,随机分为对照组、模型组和姜黄素治疗组。检测大鼠神经功能损伤评分和脑梗死体积;通过检测脑组织髓过氧物酶的含量说明中性粒细胞浸润和炎症反应的程度;检测脑组织伊文思蓝的含量说明血脑屏障的破坏程度;ELISA检测脑组织肿瘤坏死因子-α(TNF-α)的含量;免疫印记法检测基质金属蛋白酶-9(MMP-9)的表达变化。结果姜黄素治疗组减轻神经功能损伤、脑梗死体积,并减轻中性粒细胞在脑组织的浸润程度、改善血脑屏障完整性。同时姜黄素还可以降低脑组织TNF-α的含量以及MMP-9的表达水平。结论姜黄素通过减轻炎症反应和血脑屏障破坏对大鼠脑缺血/再灌注损伤起脑保护作用。姜黄素的脑保护作用可能与其降低TNF-α含量和MMP-9的表达有关。

Effects of curcumin on inflammatory reaction and blood-brain barrier permeability in rats following cerebral ischemic injury

Aim To explore the effects of curcumin on inflammatory reaction and blood-brain barrier permeability in rats following cerebral ischemic injury,and to further investigate its potential mechanisms.Methods SD rats underwent the cerebral ischemia/reperfusion injury by the sutrure occlusion model were randomly divided into sham-control,cerebral ischemia and curcumin-treatment groups.Neurological deficit scores,cerebral infarction volume,brain water content and blood-brain barrier (BBB) permeability were measured,myeloperoxidase(MPO)activities in rat brain were measured as an index of neutrophil infiltration;content of tumor necrosis factor-α(TNF-α)in rat brain was detected by ELISA;expression of matrix metalloproteinase-9(MMP-9) in rat brain was determined by Western blot.Results Neurological deficit and cerebral infarction volume was decreased in curcumin-treatment group.The degree of neutrophilicgranulo cyte infiltration in cerebral tissues was decreased and the integrity of BBB was improved.Curcumin could also inhibit TNF-α and MMP-9 expression.Conclusion Curcumin exerts the neuroprotective effect on cerebral ischemia/reperfusion injury in rats through inhibiting the inflammatory reaction and improving BBB integrity,which may be associated with the inhibiton of TNF-α and MMP-9.