Biological Properties of Dental Pulp Stem Cells Isolated from Inflamed and Healthy Pulp and Cultured in an Inflammatory Microenvironment |
| |
| Affiliation: | 1. Integral Adult Dentistry Department, Universidad de La Frontera, Temuco, Chile;2. Oral Biology Center, Faculty of Dentistry, Universidad de La Frontera, Temuco, Chile;3. Centro de Investigación en Odontología Legal y Forense (CIO), Faculty of Dentistry, Universidad de La Frontera, Temuco, Chile;1. Tandvårdens Kompetenscentrum, Norrbotten Public Dental Service, Luleå, Sweden;2. Norrbotten County Council, Luleå, Sweden, Department of Clinical Medicine and Public Health, Umeå University, Umeå, Sweden;3. Piteå Hospital, Norrbotten Public Dental Service, Piteå, Sweden;4. Department of Endodontics, Faculty of Odontology, Malmö University, Malmö, Sweden;5. Department of Odontology, Umeå University, Umeå, Sweden;1. Department of Endodontics, The University of Texas Health Science Center at Houston School of Dentistry, Houston, Texas;2. Department of Restorative Dentistry and Prosthodontics, The University of Texas Health Science Center at Houston School of Dentistry, Houston, Texas;1. Department of Endodontics, Grande Rio University, Rio de Janeiro, RJ, Brazil;2. Department of Endodontics, Fluminense Federal University, Niterói, RJ, Brazil;3. Department of Endodontics, Rio de Janeiro State University, Rio de Janeiro, RJ, Brazil;4. Department of Endodontics, Faculdade de Medicina Dentária, Universidade de Lisboa, Lisbon, Portugal;6. Unidade de Investigação em Ciências Orais e Biomédicas, Faculdade de Medicina Dentária, Universidade de Lisboa, Lisbon, Portugal;5. Centro de Estudo de Medicina Dentária Baseada na Evidência, Faculdade de Medicina Dentária, Universidade de Lisboa, Lisbon, Portugal;7. Oral Health Center, Brazilian Military Police, Unai, MG, Brazil;1. Department of Endodontics, Fluminense Federal University, Niterói, Rio de Janeiro, Brazil;2. Department of Endodontics, Feira de Santana State University, Feira de Santana, Bahia, Brazil;3. Private Practice, Changwon, Republic of Korea;4. Research Director, Cybermed, Inc, Daejeon, Republic of Korea;5. Department of Endodontics, State University of Rio de Janeiro, Rio de Janeiro, Brazil;6. Department of Dentistry II, Federal University of Maranhão, São Luís, Maranhão, Brazil;7. Dental Specialty Center, Brazilian Military Police, Minas Gerais, Brazil;1. Department of Endodontics, University of Texas Health Science Center at Houston, School of Dentistry at Houston, Houston, Texas;2. Department of Oral and Craniofacial Sciences, University of Pittsburgh, School of Dental Medicine, Pittsburgh, Pennsylvania;3. Department of Endodontics, University of Pittsburgh, School of Dental Medicine, Pittsburgh, Pennsylvania;1. Department of Endodontics, Nigde Omer Halisdemir University, Niğde, Turkey;2. Department of Oral Radiology, Faculty of Dentistry, Necmettin Erbakan University, Konya, Turkey;3. Department of Restorative Dentistry, Nigde Omer Halisdemir University, Niğde, Turkey;4. Department of Conservative Dentistry, Jordan University of science and Technology Irbid, Jordan;6. Department of Conservative Dentistry and Endodontics, University of Benghazi, Benghazi, Libya;5. International Islamic University Malaysia (IIUM), Kuala Lumpur, Malaysia;7. Poznań University of Medical Sciences Dept. of Conservative Dentistry and Endodontics Poznań, Poznań, Poland;11. Professor and Head of Endodontic Department, Al-Azhar University, Cairo, Egypt;12. Department of Odontology, School of Dentistry, Faculty of Health Sciences, University of Pretoria, Pretoria, South Africa;8. Kazakh National Medical University Asfendiyarov, Almaty, Kazakhstan;10. Department of Conservative Dentistry and Endodontics, Saveetha Dental College & Hospitals, Saveetha Institute of Medical and Technical Sciences, Saveetha University, Chennai, Tamil Nadu, India;9. Department of Conservative Dental Sciences, College of Dentistry, Qassim University, Buraydah, Qassim, Saudi Arabia;71. Department of Restorative Dental Science, Collage of Dentistry, University of Ha’il, Ha’il, Saudi Arabia;112. Department of Conservative Dentistry, Faculty of Dentistry, Thamar University, Dhamar, Yemen;123. Department of Periodontology and Operative Dentistry, Westphalian Wilhelms-University, Münster, Germany;84. Department of Endodontics and Restorative Dentistry, School of Dental Medicine, University of Zagreb, Zagreb, Croatia;106. Department of Operative Dentistry and Endodontics, Riphah International University, Pakistan;95. Center for Innovation and Research in Oral Sciences (CIROS), Faculty of Medicine, University of Coimbra, Coimbra, Portugal;77. Institute of Endodontics, Faculty of Medicine, University of Coimbra, Coimbra, Portugal;1111. Department of Endodontology, Academic Centre for Dentistry Amsterdam, University of Amsterdam and Vrije Universiteit Amsterdam, Amsterdam, The Netherlands |
| |
| Abstract: | IntroductionThe aim of this study was to assess whether the biological characteristics of dental pulp stem cells (DPSCs), such as viability, adhesion to dentin, mineralization, and release of immunomodulatory cytokines, are affected by the inflammatory status of the donor tissue and/or the sustained inflammatory environment.MethodsDPSCs were isolated from pulps from 3 caries-free teeth (healthy or hDPSCs), and from 3 teeth with irreversible pulpitis or deep caries (unhealthy DPSCs or uDPSCs). The cells were cultured in odontogenic and osteogenic media with or without lipopolysaccharides. Viability was analyzed by MTT assay at days 1, 3, 5, and 7; adhesion to dentin was evaluated through an environmental scanning electron microscope after 48 hours and through MTT assay; mineralization was analyzed with alizarin red staining after 21 days; and the release of proinflammatory (interleukin 6) and immunosuppressive cytokines (interleukin 10) was measured with the enzyme-linked immunosorbent assay after 24 hours and 7 days.ResultsThe inflammatory status of the pulp significantly reduced the viability and mineralization capacity of the DPSCs, although it did not affect the adhesion capacity to dentin or the secretion of the proinflammatory interleukin. The inflammatory microenvironment (lipopolysaccharide) only had a significant impact on the secretion of interleukin 6, which was augmented after 7 days.ConclusionsThe inflammatory status of the dental pulp should be taken into account when the use of DPSCs is intended either for research and/or for application in reparative or regenerative therapies. |
| |
| Keywords: | Dental pulp stem cells inflammation lipopolysaccharide |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
