复方胰酶散中牛奶凝固力试验的探讨

目的:建立以屎肠球菌活菌的有无和数量作为乳酶生的鉴别方法。方法:采用10-1样品溶液0.2 mL涂布于1%碳酸钙含糖牛肉琼脂培养基上,含屎肠球菌的样品,形成透明钙化圈。结果:用牛奶凝固力试验体现复方胰酶散中乳酶生量,由于胰酶对牛奶凝固力试验有消解作用,凝固现象不明确;用屎肠球菌形成透明钙化圈,直观、明确。结论:复方胰酶散中乳酶生的鉴别,采用屎肠球菌形成透明钙化圈方法代替牛奶凝固试验,方法简单,结论明确。

Discussion of milk coagulation experiment in Compound Pancreatin Powder

Abstract Objective: Method for improving identification test of milk coagulation force in compound pancreatin powder. Methods: 0.2 ml of 10-1 sample solution was applied to 1% calcium carbonate sugared beef agar medium, The sample containing enterococcus faecium forms a transparent calcification ring. Results: The milk coagulation test shows the amount of Lactasin in the compound pancreatin powder, Because of pancreatin digestion of milk coagulation force tests have effect, coagulation phenomenon is not clear; The use of Enterococcus faecium to form a transparent calcification circle is intuitive and clear. Conclusion: The identification of milk coagulation force in compound pancreatic powder, using the method of forming transparent calcification circle by Enterococcus faecium instead of milk coagulation test, the method is simple and the conclusion is clear.